Nanotube array for optical detection of protein-protein interactions
A composition can include a nanostructure, and a linker associated with the nanostructure, wherein the linker is configured to interact with a capture protein. The nanostructure can include a single-walled carbon nanotube. A plurality of the compositions can be configured in an array.
1. An array comprising a plurality of analysis regions on a substrate, at least one analysis region including a composition comprising:
a nanostructure wherein the nanostructure is a carbon nanotube having photoluminescence in near infrared wavelengths, wherein the photoluminescence in the near infrared wavelengths of the carbon nanotube is subject to quenching;
a linker associated with the nanostructure, wherein the linker has the formula A-L-C, wherein A is a polymer and the nanostructure is wrapped with the polymer, wherein the polymer is chitosan having carboxylic groups provided by activating the chitosan with succinic anhydride, wherein L-C is N α ,N α -bis(carboxymethyl)-L-Lysine and the N α ,N α -bis(carboxymethyl)-L-Lysine is directly linked via its amino group to the carboxylic group of the chitosan through an amide bond, wherein the carboxylic group containing portion of the N α ,N α -bis(carboxymethyl)-L-Lysine is a chelating region capable of associating with a nickel ion, wherein the N α ,N α -bis(carboxymethyl)-L-lysine is associated with a first binding partner through the chelating region, wherein the first binding partner is a quencher including a nickel ion; and
an analyte-interacting capture protein including a second binding partner that binds to the first binding partner.
2. The array of claim 1 , wherein the second binding partner is a protein tag.
3. The array of claim 1 , wherein the capture protein is a lectin and the analyte includes a glycan.
4. The array of claim 1 , wherein at least one analysis region further comprises a sample.
5. The array of claim 4 , wherein the sample includes a protein.
6. The array of claim 4 , the plurality of analysis regions comprising two or more subsets of analysis regions.
7. The array of claim 6 , wherein an Nth subset of analysis regions includes an Nth composition including an Nth capture protein, wherein N is an integer between 1 and 5000.
8. The array of claim 6 , wherein the composition further comprises a capture protein and an Nth subset of analysis regions includes an Nth sample, wherein N is an integer between 1 and 5000.
9. The array of claim 6 , wherein the composition further comprises a capture protein and an Nth subset of analysis regions includes an Nth analyte, wherein N is an integer between 1 and 5000.
10. A composition comprising:
a nanostructure wherein the nanostructure is a carbon nanotube having photoluminescence in near infrared wavelengths, wherein the photoluminescence in the near infrared wavelengths of the carbon nanotube is subject to quenching;
a linker associated with the nanostructure, wherein the linker has the formula A-L-C, wherein A is a polymer and the nanostructure is wrapped with the polymer, wherein the polymer is chitosan having carboxylic groups provided by activating the chitosan with succinic anhydride, wherein L-C is N α ,N α -bis(carboxymethyl)-L-Lysine and the N α ,N α -bis(carboxymethyl)-L-Lysine is directly linked via its amino group to the carboxylic group of the chitosan through an amide bond, wherein the carboxylic group containing portion of the N α ,N α -bis(carboxymethyl)-L-Lysine is a chelating region capable of associating with a nickel ion, wherein the N α ,N α -bis(carboxymethyl)-L-lysine is associated with a first binding partner through the chelating region, wherein the first binding partner is a quencher including a nickel on; and
an analyte-interacting capture protein including a second binding partner that binds to the first binding partner.