Interleukin-1 alpha antibodies and methods of use
Fully human monoclonal Abs includes (i) an antigen-binding variable region that exhibits very high binding affinity for IL-1α and (ii) a constant region that is effective at both activating the complement system though C1q binding and binding to several different Fc receptors.
1. A method of inhibiting migration of a cell through a basement membrane matrix, the method comprising the step of adding a purified mAb that specifically binds to human IL-1α to a mixture comprising the basement membrane matrix and the cell,
wherein the purified mAb is a human IgG1 mAb comprising a heavy chain covalently joined to a light chain; and
wherein the heavy chain comprises the amino acid sequence of SEQ ID NO: 9 and a light chain comprises the amino acid sequence of SEQ ID NO:11.