Interleukin-1 Alpha Antibodies and Methods of Use
Fully human monoclonal Abs includes (i) an antigen-binding variable region that exhibits very high binding affinity for IL-1α and (ii) a constant region that is effective at both activating the complement system though C1q binding and binding to several different Fc receptors.
1 . A method of inhibiting an IL-1α induced increase in expression of ICAM-1 or E-selectin on the surface of a human endothelial cell, the method comprising the step of adding a purified mAb that specifically binds to human IL-1α to a mixture comprising the endothelial cell and IL-1α.
2 . The method of claim 1 , wherein the purified mAb is a human IgG1 mAb comprising a heavy chain covalently joined to a light chain.
3 . The method of claim 2 , wherein the heavy chain comprises the amino acid sequence of SEQ ID NO: 9 and a light chain comprises the amino acid sequence of SEQ ID NO:11.