Interleukin-1 Alpha Antibodies and Methods of Use
Fully human monoclonal Abs includes (i) an antigen-binding variable region that exhibits very high binding affinity for IL-1α and (ii) a constant region that is effective at both activating the complement system though C1q binding and binding to several different Fc receptors.
1 . A method of tracking inflammation in a human subject previously subjected to the steps of: obtaining from the subject a first sample of peripheral blood mononuclear cells at a first time; contacting the first sample with a purified mAb that specifically binds to human IL-1α; and determining the percent of cells in the first sample that bind the mAb, the method comprising the steps of:
(a) obtaining from the subject a second sample of peripheral blood mononuclear cells at a second time;
(b) contacting the second sample with the purified mAb that specifically binds to human IL-1α;
(c) determining the percent of cells in the second sample that bind the monoclonal Ab; and
(d) comparing the percent of cells in the first sample that bind the mAb to the percent of cells in the second sample that bind the monoclonal Ab.
2 . The method of claim 1 , wherein the purified mAb is a human IgG1 mAb comprising a heavy chain covalently joined to a light chain.
3 . The method of claim 2 , wherein the heavy chain comprises the amino acid sequence of SEQ ID NO: 9 and a light chain comprises the amino acid sequence of SEQ ID NO:11.