Porin B (PorB) as a therapeutic target for prevention and treatment of infection by
View Patent ↗The present invention features peptides of a PorB polypeptide, which PorB peptides are useful in production of antibodies that bind the full-length PorB polypeptide and as a therapeutic agent. In specific embodiments the invention features a composition comprising one or more PorB peptides (other than a full-length PorB polypeptide), which peptides contain at least one epitope that can elicit Chlamydia -neutralizing antibodies. The invention also features methods for induction of a protective immune response against infection by Chlamydia and Chlamydiophila.
1. A composition comprising:
an isolated polypeptide up to 60 amino acids in length, wherein the isolated polypeptide contains an amino acid sequence of SEQ ID NO: 12 (QKNACSFDLCNSYDVL), or an amino acid sequence of SEQ ID NO: 12 comprising at least one conservative amino acid substitution, wherein the isolated polypeptide binds to neutralizing C. trachomatis PorB polypeptide-specific antisera; and
a pharmaceutically acceptable carrier.
2. The composition of claim 1 , wherein the isolated polypeptide contains the amino acid sequence of SEQ ID NO: 12.
3. The composition of claim 1 , wherein the isolated polypeptide contains the amino acid sequence of SEQ ID NO: 12 comprising at least one conservative amino acid substitution.
4. The composition of claim 2 , wherein the isolated polypeptide is a fusion polypeptide comprising a non-PorB amino acid sequence and the amino acid sequence of SEQ ID NO:12.
5. The composition of claim 3 , wherein the isolated polypeptide is a fusion polypeptide comprising a non-PorB amino acid sequence and the amino acid sequence of SEQ ID NO:12 comprising at least one conservative amino acid substitution.
6. The composition of claim 1 , wherein the isolated polypeptide is a polypeptide of amino acid sequence of SEQ ID NO:12.
7. The composition of claim 1 , wherein the isolated polypeptide is a polypeptide of amino acid sequence of SEQ ID NO:12 comprising at least one conservative amino acid substitution.
8. The composition of claim 1 , wherein the isolated polypeptide is a polypeptide of amino acid sequence of SEQ ID NO: 7 (FPVIPGINIEQKNACSFDLCNSYDVL).
9. The composition of claim 1 , wherein the isolated polypeptide comprises at least 30 amino acids.
10. The composition of claim 1 , wherein the isolated polypeptide comprises at least 40 amino acids.
11. The composition of claim 1 , wherein the isolated polypeptide comprises at least 50 amino acids.
12. The composition of claim 1 , wherein the composition additionally comprises at least one PorB polypeptide with an amino acid sequence different from the amino acid sequence of SEQ ID NO: 12 or SEQ ID NO: 12 comprising at least one conservative amino acid substitution.
13. The composition of claim 11 , wherein the composition additionally comprises at least one PorB polypeptide with an amino acid sequence selected from the group consisting of: SEQ ID NO:8 (ND2), SEQ ID NO:9 (ND3), and SEQ ID NO:10 (ND4).
14. The composition of claim 1 , wherein the composition additionally comprises at least one PorB polypeptide with an amino acid sequence selected from the group consisting of: SEQ ID NO:11 (B1-2), B2-3 (SEQ ID NO:13), SEQ ID NO:14 (B2-4), SEQ ID NO:15 (B3-2), SEQ ID NO:16 (B3-3), SEQ ID NO:17 (B3-4), SEQ ID NO:18 (B4-4), SEQ ID NO:19 (B5-1), and SEQ ID NO:20 (B5-2).
15. A composition comprising:
an isolated C. trachomatis PorB polypeptide up to 60 amino acids in length containing an amino acid sequence of SEQ ID NO: 12 (QKNACSFDLCNSYDVL), or an amino acid sequence of SEQ ID NO: 12 comprising at least one conservative amino acid substitution, wherein the isolated polypeptide binds to neutralizing C. trachomatis PorB polypeptide-specific antisera; and
a pharmaceutically acceptable carrier.
16. The composition of claim 15 , wherein the isolated polypeptide contains the amino acid sequence of SEQ ID NO: 12.
17. The composition of claim 15 , wherein the isolated polypeptide contains the amino acid sequence of SEQ ID NO: 12 comprising at least one conservative amino acid substitution.
18. The composition of claim 15 , wherein the isolated polypeptide is a polypeptide of amino acid sequence of SEQ ID NO: 12 (QKNACSFDLCNSYDVL).
19. The composition of claim 15 , wherein the isolated polypeptide is a polypeptide of amino acid sequence of SEQ ID NO:12 comprising at least one conservative amino acid substitution.
20. The composition of claim 15 , wherein the composition additionally comprises at least one PorB polypeptide with an amino acid sequence different from the amino acid sequence of SEQ ID NO: 12 or SEQ ID NO:12 comprising at least one conservative amino acid substitution.