IP Library › Granted Patent US 8,492,144
Granted Patent B2
US 8,492,144 · App. 13/232,462 · Granted Jul 23, 2013

Methods for improving viability and productivity in cell culture

Inventors: Haimanti Dorai (Radnor, PA); Celia Ly (Malvern, PA); Tina M. Sauerwald McClain (Royersford, PA)
Assignee: Janssen Biotech, Inc.
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Quick Facts
Patent No.
US 8,492,144
App. No.
13/232,462
Granted
Jul 23, 2013
Kind
B2
Abstract

Methods for increasing viability and production of secreted proteins in fed batch eukaryotic cell culture are disclosed.

Claims (4)

1. A method of producing a secreted protein in a Chinese Hamster Ovary (CHO) fed batch cell culture, comprising culturing a CHO cell line over-expressing MDM2 and E1B19K and one or more genes encoding the secreted protein, wherein the titer of the produced secreted protein is at least 600 mg/L at day 23 of the fed batch culture.

2. The method of claim 1 wherein the CHO cell line is CHO-K1.

3. The method of claim 1 wherein the CHO cell line is CHO-K1SV.

4. The method of claim 1 wherein the secreted protein is an antibody heavy chain and an antibody light chain.

Assignments (1)
CHANGE OF NAME Recorded Jun 6, 2013
From: CENTOCOR ORTHO BIOTECH INC.
To: JANSSEN BIOTECH, INC.
Reel/Frame 030573/0464 →
Continuity (3)
Division 12483658 · Jun 12, 2009
Provisional Application 61061235 · Jun 13, 2008
Related Publication 20120003735A1 · Jan 5, 2012