Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. An isolated polynucleotide that encodes a polypeptide having cellulolytic enhancing activity, wherein the polypeptide
comprises an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.
2. The isolated polynucleotide of claim 1 , that encodes a polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 2.
3. The isolated polynucleotide of claim 1 , wherein the mature polypeptide is amino acids 16 to 317 of SEQ ID NO: 2.
4. A nucleic acid construct comprising the polynucleotide of claim 1 operably linked to one or more control sequences that direct the production of the polypeptide in an expression host.
5. A recombinant expression vector comprising the nucleic acid construct of claim 4 .
6. A recombinant host cell comprising the nucleic acid construct of claim 4 .
7. A method of producing a polypeptide having cellulolytic enhancing activity, comprising: (a) cultivating the host cell of claim 6 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
8. A method of producing a mutant of a parent cell, comprising disrupting or deleting a polynucleotide sequence that encodes a polypeptide having cellulolytic enhancing activity and at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2, which results in the mutant producing less of the polypeptide than the parent cell.
9. A method of producing a polypeptide having cellulolytic enhancing activity, comprising: (a) cultivating a transgenic plant or a plant cell comprising the polynucleotide of claim 1 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
10. A transgenic plant, plant part or plant cell transformed with the polynucleotide of claim 1 .
11. A nucleic acid construct comprising a gene encoding a protein operably linked to a nucleotide sequence encoding a signal peptide comprising or consisting of amino acids 1 to 15 of SEQ ID NO: 2, wherein the gene is foreign to the nucleotide sequence.
12. A recombinant host cell comprising the nucleic acid construct of claim 11 .
13. A method of producing a protein, comprising: (a) cultivating the recombinant host cell of claim 12 under conditions conducive for production of the protein; and
(b) recovering the protein.
14. The isolated polynucleotide of claim 1 , that encodes a polypeptide having at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2.
15. The isolated polynucleotide of claim 1 , that encodes a polypeptide having at least 98% sequence identity to the mature polypeptide of SEQ ID NO: 2.
16. The isolated polynucleotide of claim 1 , that encodes a polypeptide having at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 2.
17. The isolated polynucleotide of claim 3 , comprising or consisting of the nucleotide sequence of SEQ ID NO: 1.
18. An isolated polynucleotide that encodes a polypeptide having cellulolytic enhancing activity, selected from the group consisting of:
(a) a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii); and
(b) a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.
19. The isolated polynucleotide of claim 18 , that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii).
20. The isolated polynucleotide of claim 18 , that hybridizes under at least very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii).
21. The isolated polynucleotide of claim 18 , comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.
22. The isolated polynucleotide of claim 18 , comprising a nucleotide sequence having at least 97% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.
23. The isolated polynucleotide of claim 18 , comprising a nucleotide sequence having at least 98% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 .
24. The isolated polynucleotide of claim 18 , comprising a nucleotide sequence having at least 99% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 .
25. The isolated polynucleotide of claim 18 , wherein the mature polypeptide coding sequence is nucleotides 46 to 951 of SEQ ID NO: 1.
26. The method of claim 8 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 2.
27. The method of claim 8 , wherein the polynucleotide sequence comprises the nucleotide sequence of SEQ ID NO: 1.