Storage-induced promoter
A promoter with an organ-specific activity in plants. The promoter is characterized in that it exhibits greater activity in the storage organs of plants than in other organs of said plants and that the promoter activity is modified after the harvest of the storage organs and is greater than prior to said harvest.
1. A vector or mobile genetic element including a promoter comprising:
the nucleotide sequence according to SEQ ID NO: 4, wherein the promoter is operably linked to a nucleic acid, and wherein said nucleic acid is heterologous relative to said promoter.
2. A plant or prokaryotic host cell including the vector or genetic element according to claim 1 .
3. A transgenic plant or parts thereof transformed with the vector or genetic element according to claim 1 .
4. The transgenic plant according to claim 3 , wherein said plant is Beta vulgaris.
5. A transgenic plant or prokaryotic host cell including a promoter comprising:
the nucleotide sequence according to SEQ ID NO: 4 wherein the promoter is operably linked to a nucleic acid, and wherein said nucleic acid is heterologous relative to said promoter.
6. A transgenic plant or parts thereof transformed with a nucleic acid comprising the nucleotide sequence according to SEQ ID NO: 4.
7. The transgenic plant according to claim 6 , wherein said plant is Beta vulgaris.
8. A method for producing a transgenic plant, said method comprising transforming a plant with a promoter comprising the nucleotide sequence according to SEQ ID NO: 4 operably linked to a coding sequence for producing a transgenic plant with one or more of the following properties: a) improved carbohydrate metabolism in the storage organs after harvest, b) improved nitrogen metabolism in the storage organs after harvest, c) improved dry stress resistance and improved water status in the storage organs after harvest, d) improved cold and frost tolerance of the storage organs after harvest, e) increased resistance/tolerance of the storage organs against pathogens after harvest, or f) improved secondary metabolism in the storage organs after harvest.
9. The vector or mobile genetic element according to claim 1 , wherein the activity of the promoter in the storage organs post harvest is measurable through RNA-blot, which in comparable test conditions prior to the harvest of the storage organs is detectable at less than 20% of the activity post harvest.
10. The vector or mobile genetic element according to claim 1 , wherein the activity of the promoter in the storage organs post harvest is measurable through RNA-blot, which in comparable test conditions prior to the harvest of the storage organs is detectable at less than 10% of the activity post harvest.
11. The vector or mobile genetic element according to claim 1 , wherein the activity of the promoter in the storage organs post harvest is measurable through RNA-blot, which in comparable test conditions prior to the harvest of the storage organs is detectable at less than 5% of the activity post harvest.
12. The plant or prokaryotic host cell according to claim 5 , wherein the activity of the promoter in the storage organs post harvest is measurable through RNA-blot, which in comparable test conditions prior to the harvest of the storage organs is detectable at less than 20% of the activity post harvest.
13. The plant or prokaryotic host cell according to claim 5 , wherein the activity of the promoter in the storage organs post harvest is measurable through RNA-blot, which in comparable test conditions prior to the harvest of the storage organs is detectable at less than 10% of the activity post harvest.
14. The plant or prokaryotic host cell according to claim 5 , wherein the activity of the promoter in the storage organs post harvest is measurable through RNA-blot, which in comparable test conditions prior to the harvest of the storage organs is detectable at less than 5% of the activity post harvest.
15. The transgenic plant or parts thereof according to claim 6 , wherein the activity of the promoter in the storage organs post harvest is measurable through RNA-blot, which in comparable test conditions prior to the harvest of the storage organs is detectable at less than 20% of the activity post harvest.
16. The transgenic plant or parts thereof according to claim 6 , wherein the activity of the promoter in the storage organs post harvest is measurable through RNA-blot, which in comparable test conditions prior to the harvest of the storage organs is detectable at less than 10% of the activity post harvest.
17. The transgenic plant or parts thereof according to claim 6 , wherein the activity of the promoter in the storage organs post harvest is measurable through RNA-blot, which in comparable test conditions prior to the harvest of the storage organs is detectable at less than 5% of the activity post harvest.
18. The method according to claim 8 , wherein the activity of the promoter in the storage organs post harvest is measurable through RNA-blot, which in comparable test conditions prior to the harvest of the storage organs is detectable at less than 20% of the activity post harvest.
19. The method according to claim 8 , wherein the activity of the promoter in the storage organs post harvest is measurable through RNA-blot, which in comparable test conditions prior to the harvest of the storage organs is detectable at less than 10% of the activity post harvest.
20. The method according to claim 8 , wherein the activity of the promoter in the storage organs post harvest is measurable through RNA-blot, which in comparable test conditions prior to the harvest of the storage organs is detectable at less than 5% of the activity post harvest.