Method for obtaining inulin from plants
View Patent ↗The invention describes a method for obtaining inulin which comprises: a) finely comminuting inulin-containing plant material, wherein particle sizes of the plant material are generated which are smaller than the size of the inulin-containing plant cells, b) suspending the finely comminuted plant material in a liquid, wherein inulin is released from the plant material and suspended in particulate form in the liquid, c) separating off plant particles from the liquid, wherein the inulin which is suspended in particulate form remains in the liquid, d) separating off the inulin which is suspended in particulate form from the liquid. The method is suitable in particular for obtaining inulin from artichoke roots.
1. A method for obtaining inulin comprising:
a) finely comminuting inulin-containing plant material, wherein the comminuting produces plant particles that are smaller in size than inulin-containing plant cells,
b) suspending the finely comminuted plant particles in a liquid thereby forming a suspension, and releasing inulin from the plant particles, in particulate form into the liquid,
c) separating plant particles from the liquid, wherein the inulin which is suspended in particulate form remains in the liquid, which is carried out at a temperature of 5° C. to <60° C., and
d) separating off the inulin which is suspended in particulate form from the liquid.
2. The method according to claim 1 , further comprising purifying the inulin from d).
3. The method according to claim 2 , wherein the purification is done using recrystallization, (ultra)filtration, dissolution and precipitation with a solvent, or chromatography.
4. The method according to claim 2 , wherein the fine comminution is achieved by pureeing, and wherein the liquid of step (b) is water.
5. The method according to claim 4 , wherein the plant material is artichoke roots ( Cynara scolymus or Cynara cardunculus L.) or chicory roots ( Cichorium intybus ).
6. The method according to claim 2 , wherein steps b) and c) are carried out at the same temperature.
7. The method according to claim 1 , wherein the liquid of step b) is water.
8. The method according to claim 1 , wherein the suspension of step b) has a temperature of 5° C. to <60° C.
9. The method according to claim 8 , wherein step c) is carried out at a temperature of 5° C. to <60° C., and wherein step d) is carried out at a temperature of 5° C. to <60° C.
10. The method according to claim 1 , wherein the plant material is plant roots, tubers, stems, blossoms, or leaves.
11. The method according to claim 10 , wherein the plant roots are artichoke roots ( Cynara scolymus or Cynara cardunculus L.) or chicory roots ( Cichorium intybus ).
12. The method according to claim 11 , wherein the plant roots are artichoke roots, and further comprising obtaining inulin from said roots having a weight average degree of polymerization of 40 to 80 DPw, as determined by GPC-RI-MALLS.
13. The method according to claim 11 , wherein the plant roots are artichoke roots, and further comprising obtaining inulin from said roots having a weight average degree of polymerization of 50 to 80 DPw, as determined by GPC-RI-MALLS.
14. The method according to claim 13 , wherein the inulin has a number average degree of polymerization of 41 to 66.
15. The method according to claim 11 , wherein the plant roots are artichoke roots, and further comprising obtaining inulin from said roots having a number average degree of polymerization of 41 to 66.
16. The method according to claim 1 , comprising separating the plant particles of step c) from the liquid by a decanter or a sieve.
17. The method according to claim 1 , comprising separating the suspended particulate form inulin molecules from the liquid by centrifugation.
18. The method according to claim 1 , wherein the fine comminution is achieved by pureeing the plant material.
19. The method according to claim 1 , wherein the inulin obtained in step d) has a chain length (DP) greater than 10.
20. The method according to claim 1 , wherein the plant particles produced by fine comminuting have disrupted plant cells.
21. The method according to claim 1 , wherein the liquid of step b) is water, and wherein the plant material is artichoke roots ( Cynara scolymus or Cynara cardunculus L.) or chicory roots ( Cichorium intybus ).
22. The method according to claim 1 , wherein steps b) and c) are carried out at the same temperature.
23. The method according to claim 1 , wherein steps b), c), and d) are carried out at the same temperature.
24. The method according to claim 1 , wherein the inulin obtained has a weight average degree of polymerization (DPw) between 40 and 80, as determined by GPC-RI-MALLS.
25. The method according to claim 1 , wherein the inulin obtained has a weight average degree of polymerization (DPw) between 50 and 80, as determined by GPC-RI-MALLS.
26. The method according to claim 1 , wherein the suspension of step b) has a temperature of 15 to 30° C., wherein step c) is carried out at a temperature of 15 to 30° C., and wherein step d) is carried out at a temperature of 15 to 30° C.
27. The method according to claim 1 , wherein the suspension of step b) has a temperature of 20 to 30° C., wherein step c) is carried out at a temperature of 20 to 30° C., and wherein step d) is carried out at a temperature of 20 to 30° C.
28. The method according to claim 1 , wherein the suspension of step b) has a temperature of 20 to 25° C., wherein step c) is carried out at a temperature of 20 to 25° C., and wherein step d) is carried out at a temperature of 20 to 25° C.