NEIL3 peptides and vaccines including the same
The present invention provides isolated peptides or the fragments derived from SEQ ID NO: 45, which bind to an HLA antigen and induce cytotoxic T lymphocytes (CTL). The peptides may include the above mentioned amino acid sequence with substitution deletion, or addition of one, two, or several amino acids sequences. The invention also provides pharmaceutical compositions including these peptides. The peptides of this invention can be used for diagnosing or treating cancer.
1. A method of inducing an immune response against cancer overexpressing NEIL3 in a subject, the method comprising administering to the subject a composition comprising a peptide selected from the group consisting of:
(a) an isolated peptide of less than 15 amino acids comprising the amino acid sequence of SEQ ID NO: 5;
(b) an isolated peptide consisting of an amino acid sequence of SEQ ID NO: 5, in which 1 or 2 amino acid(s) are substituted, deleted, or added, wherein the peptide has CTL inducibility.
2. The method of claim 1 , wherein the cancer is selected from the group consisting of bladder cancer, breast cancer, cervical cancer, cholangiocellular carcinoma, colorectal cancer, endometriosis, esophagus cancer, liver cancer, NSCLC, osteosarcoma, pancreatic cancer, prostate cancer, renal carcinoma, SCLC, soft tissue tumor, AML and CML.
3. The method of claim 1 , wherein the wherein the dosage of the peptide is 0.1 mg to 10 mg.
4. The method of claim 1 , wherein the peptide is administered in combination with an adjuvant.
5. The method of claim 4 , wherein the adjuvant is incomplete Freund's adjuvant.
6. The method of claim 1 , wherein the peptide is administered by subcutaneous injection.
7. The method of claim 1 , wherein the peptide consists of the amino acid sequence of SEQ ID NO: 5.
8. The method of claim 1 , wherein the peptide has one or both of the following characteristics:
(a) the second amino acid from the N-terminus of the amino acid sequence of SEQ ID NO: 5 is substituted with leucine or methionine; and
(b) the C-terminal amino acid of the amino acid sequence of SEQ ID NO: 5 is substituted with leucine.