Manipulation of particles in channels
Systems and methods for the manipulation of particles within channels such as microfluidic channels are provided. In one set of embodiments, magnets are positioned around a channel. As a fluid containing magnetic and non-magnetic particles flows through the channel, the magnetic field created by the magnets can be used to transport the magnetic and/or non-magnetic particles to desired locations within the channel, which may useful in some cases for causing some separation of the particles. For example, the magnetic field may be used to transport magnetic or non-magnetic particles from a core fluid to a surrounding sheath fluid. In some cases, the magnetic field is used to transport non-magnetic particles to a small volume within the channel (e.g., a single-file row within the channel). The systems and methods described herein may find application in a variety of fields including, for example, continuous sorting of cells, removal of targeted cells from a stream of blood, or the arrangement of non-magnetic particles in channels for analysis.
1. A method, comprising:
applying a magnetic field to a channel containing a core fluid and a cladding fluid surrounding the core fluid, the cladding fluid being different from the core fluid and contacting the core fluid at an interface, at least one of the core and cladding fluids containing magnetic particles, wherein at least some of the magnetic particles cross the interface due to the applied magnetic field.
2. The method of claim 1 , further comprising flowing the core and cladding fluids through the channel.
3. The method of claim 1 , wherein the channel is a microchannel.
4. The method of claim 1 , further comprising separating the core fluid from the cladding fluid.
5. The method of claim 4 , further comprising separating the core fluid from the cladding fluid downstream of the magnetic field.
6. The method of claim 1 , wherein the magnetic field is generated by a permanent magnet.
7. The method of claim 1 , wherein the magnetic field is generated by an electromagnet.
8. The method of claim 1 , wherein the magnetic field is arranged such that a magnetic field minimum is positioned substantially along a center axis of the channel, the center axis being defined in a direction of fluid flow within the channel.
9. The method of claim 1 , wherein the magnetic field is applied to a first portion of the channel, the method further comprising applying a second magnetic field to a second portion of the channel different from the first portion of the channel.
10. The method of claim 9 , wherein the second magnetic field has a magnetic field distribution that is different from that of the first magnetic field.
11. The method of claim 1 , wherein at least some of the magnetic particles are fastened to cells.
12. The method of claim 1 , wherein the core fluid and/or cladding fluid comprises saline.
13. The method of claim 1 , wherein the core fluid and/or cladding fluid comprises blood.
14. The method of claim 1 , wherein the core fluid comprises blood and the cladding fluid comprises saline.
15. The method of claim 1 , further comprising providing a third fluid surrounding the cladding fluid, the third fluid being different from the cladding fluid and contacting the cladding fluid at a second interface.
16. The method of claim 15 , further comprising separating the core fluid, the cladding fluid, and the third fluid.
17. The method of claim 1 , wherein at least some of the magnetic particles are transported from the core fluid to the cladding fluid due to the applied magnetic field.
18. The method of claim 1 , wherein at least some of the magnetic particles are transported from the cladding fluid to the core fluid due to the applied magnetic field.
19. The method of claim 1 , wherein at least one of the core and cladding fluids contains non-magnetic particles.
20. The method of claim 19 , wherein the cladding fluid contains non-magnetic particles.
21. The method of claim 20 , wherein at least some of the non-magnetic particles are transported from the cladding fluid to the core fluid due to the applied magnetic field.
22. The method of claim 1 , wherein the core fluid and the cladding fluid are immiscible.