IP Library › Patent Application 13262978
Patent Application
App. No. 13/262,978

ENHANCED CELLULASE EXPRESSION IN S. DEGRADANS

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Quick Facts
Patent No.
US None
App. No.
13/262,978
Abstract

The invention provides organisms and methods of using and making organisms with enhanced cellulase expression.

Claims (17)

1 . A genetically modified host cell, wherein said host cell is genetically modified with one or more isolated nucleic acids selected from the group consisting of:

a. a polynucleotide having a sequence of Cel5A, Cel5G, Cel5H, or Cel5J;

b. a polynucleotide encoding a polypeptide having a sequence of Cel5A, Cel5G, Cel5H, or Cel5J;

c. a polynucleotide having at least 95% sequence identity to a polynucleotide having a sequence of Cel5A, Cel5G, Cel5H, or Cel5J, wherein the polynucleotide encodes a polypeptide having processive endoglucanase activity;

d. a polynucleotide encoding a polypeptide having at least 95% sequence identity to a polypeptide having a sequence of that encodes any of Cel5A, Cel5G, Cel5H, or Cel5J, wherein the polynucleotide encodes a polypeptide having processive endoglucanase activity; and

e. a polynucleotide that hybridizes under stringent conditions to the complement of any of the polynucleotides of a) through d) above, wherein the polynucleotide encodes a polypeptide having processive endoglucanase activity.

2 . The genetically modified host cell of claim 1 , wherein the expression of the polynucleotide in the host cell results in increased yield of cellobiose in the presence of cellulosic material.

3 . The genetically modified host cell of claim 1 , wherein the expression of the polynucleotide in the host cell is at least 50-fold over control levels.

4 . The genetically modified host cell of claim 1 , wherein the host cell is Saccharophagus degradans.

5 . A method of producing a genetically modified host cell containing an isolated nucleic acid encoding a polypeptide, wherein the method comprises the steps of transforming a host cell with an expression vector comprising the nucleic acid, wherein the nucleic acid comprises a polynucleotide selected from the group consisting of:

a. a polynucleotide having a sequence that encodes any of Cel5A, Cel5G, Cel5H, or Cel5J;

b. a polynucleotide encoding a polypeptide having a sequence of Cel5A, Cel5G, Cel5H, or Cel5J;

c. a polynucleotide having at least 95% sequence identity to a polynucleotide having a sequence as that encodes any of Cel5A, Cel5G, Cel5H, or Cel5J, wherein the polynucleotide encodes a polypeptide having processive endoglucanase activity;

d. a polynucleotide encoding a polypeptide having at least 95% sequence identity to a polypeptide having a sequence of Cel5A, Cel5G, Cel5H, or Cel5J, wherein the polynucleotide encodes a polypeptide having processive endoglucanase activity; and

e. a polynucleotide that hybridizes under stringent conditions to the complement of any of the polynucleotides of a) through d) above, wherein the polynucleotide encodes a polypeptide having processive endoglucanase activity.

6 . The method of claim 5 , wherein the nucleic acid is operably linked to one or more regulatory sequences.

7 . The method of claim 5 , wherein the regulatory sequence is a promoter.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 26, 2015
From: HUTCHESON, STEVEN
To: UNIVERSITY OF MARYLAND, COLLEGE PARK
Reel/Frame 034814/0245 →
SECURITY AGREEMENT Recorded Jul 13, 2012
From: AEMETIS TECHNOLOGIES, INC.
To: THIRD EYE CAPITAL CORPORATION
Reel/Frame 028554/0362 →