DNA-cell conjugates
The present invention provides conjugates of DNA and cells by linking the DNA to a native functional group on the cell surface. The cells can be without cell walls or can have cell walls. The modified cells can be linked to a substrate surface and used in assay or bioreactors.
1. A composition comprising: a live cell that has no cell wall, wherein the cell has a surface comprising a native functional group linked to a nucleic acid moiety, wherein the nucleic acid moiety is covalently linked directly to the native functional group, wherein the native functional group comprises an amino acid selected from the group consisting of lysine, cysteine, tyrosine, threonine, serine, aspartic acid, glutamic acid and tryptophan, and wherein the cell has not undergone metabolic engineering to introduce an azide modified sugar.
2. The composition of claim 1 , wherein the cell is a primary cell.
3. The composition of claim 1 , wherein the cell is a mammalian cell.
4. The composition of claim 1 , wherein the cell is a stem cell.
5. The composition of claim 1 , wherein the native functional group comprises lysine.
6. The composition of claim 1 , wherein the nucleic acid moiety comprises a member selected from the group consisting of an oligonucleotide, DNA, RNA, PNA and an aptamer.
7. The composition of claim 1 , wherein the nucleic acid moiety comprises single-stranded DNA.
8. The composition of claim 1 , wherein the nucleic acid moiety comprises from about 10 to about 200 nucleic acids.
9. The composition of claim 1 , wherein the nucleic acid moiety comprises an aptamer.
10. The composition of claim 1 , wherein the nucleic acid moiety comprises a linker.
11. The composition of claim 1 , comprising a mammalian cell comprising lysine on the cell surface; and a single-stranded deoxy-nucleic acid covalently linked to the lysine via an amide.
12. A composition comprising: a live cell that has no cell wall, wherein the cell has a surface comprising a native functional group linked to a nucleic acid moiety, wherein the native functional group comprises an amino acid selected from the group consisting of lysine, cysteine, tyrosine, threonine, serine, aspartic acid, glutamic acid and tryptophan, and the nucleic acid moiety is covalently linked to the native functional group.
13. The composition of claim 12 , wherein the cell is a primary cell.
14. The composition of claim 12 , wherein the cell is a mammalian cell.
15. The composition of claim 12 , wherein the cell is a stem cell.
16. The composition of claim 12 , wherein the native functional group comprises lysine.
17. The composition of claim 12 , wherein the nucleic acid moiety comprises a member selected from the group consisting of an oligonucleotide, DNA, RNA, PNA and an aptamer.
18. The composition of claim 12 , wherein the nucleic acid moiety comprises single-stranded DNA.
19. The composition of claim 12 , wherein the nucleic acid moiety comprises a linker.
20. The composition of claim 12 , comprising a mammalian cell comprising lysine on the cell surface; and a single-stranded deoxy-nucleic acid covalently linked to the lysine via an amide.
21. A composition comprising: a live cell that has no cell wall, wherein the cell has a surface comprising a native functional group linked to a nucleic acid moiety, wherein the native functional group comprises an amino acid, and the nucleic acid moiety is linked to the native functional group by a covalent bond formed by reaction with a primary amine of the native functional group.