Carbohydrate degrading polypeptide and uses thereof
The invention relates to a polypeptide having carbohydrate material degrading activity which comprises the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 4, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.
1. A variant polypeptide having at least 96% sequence identity with the sequence set out in SEQ ID NO:2 or the mature form thereof, wherein said variant polypeptide or mature form thereof has one or more substitutions or insertions relative to SEQ ID NO:2, and wherein the polypeptide has beta-cellobiohydrolase activity.
2. The polypeptide according to claim 1 , wherein the polypeptide has carbohydrate material degrading activity against carbohydrate material comprising lignocellulose, wherein the carbohydrate material is optionally pretreated.
3. A process for the preparation of a fermentation product, which process comprises:
a) degrading lignocellulose by contacting the lignocellulose with a polypeptide having at least 96% sequence identity with the sequence set out in SEQ ID NO:2, or the mature form thereof; or a composition comprising a polypeptide having at least 96% sequence identity with the sequence set out in SEQ ID NO:2, or the mature form thereof, and a cellulase and/or a hemicellulase and/or a pectinase,
b) fermenting the resulting material to prepare a fermentation product, and
c) optionally, recovering the fermentation product.
4. The process of claim 3 , wherein the fermentation product is recovered in step (c), and the fermentation product is ethanol.