Method for diagnosing a hemoglobin-related disorder
A method for diagnosing and/or staging a hemoglobin disorder such as β-thalassemia involves contacting a blood sample of a subject with an Alpha-Hemoglobin Stabilizing Protein which may be present on a solid support, and detecting and/or quantifying free α-Hb. Detection or quantification can be achieved by photometry such as HTRF or immunological procedures such as EIA and ELISA. The free α-Hb is compared to a reference value, and can be used to correlate with the diagnosis and/or staging of the hemoglobin disorder for the subject.
1. A method for diagnosing and/or staging β-thalassemia in a subject in need thereof, said method comprising:
contacting a blood sample obtained from said subject with Alpha-Hemoglobin Stabilizing Protein (AHSP),
detecting and/or quantifying the presence of free monomeric α-Hb in said blood sample by using at least one labelled antibody that binds specifically to α-Hb,
comparing an amount of free monomeric α-Hb detected and/or quantified in said detecting and/or quantifying step with a reference value, wherein said reference value comprises the amount of free monomeric α-Hb detected and/or quantified in at least one control subject not diagnosed with a hemoglobin disorder; and
correlating said amount of free monomeric α-Hb with the diagnosis and/or the staging of a hemoglobin disorder in said subject, wherein significantly higher amounts of said free monomeric α-Hb detected and/or quantified in said subject as compared to said reference value corresponds to the severity of β-thalassemia in said subject.