Methods and compositions for protein labeling using lipoic acid ligases
The present disclosure provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ lipoic acid ligase or mutants thereof, and lipoic acid analogs (e.g., lipoic acid analogs comprising a resorufin moiety) recognized by lipoic acid ligase and lipoic acid ligase mutants. Also provided herein is a method of imaging protein-protein interaction via a reaction mediated by lipoic acid ligase.
1. An isolated lipoic acid ligase mutant that uses resorufin as a substrate, the mutant comprising an amino acid sequence that is at least 85% identical to SEQ ID NO:11 and includes mutations at residues corresponding to E20 and F147 in SEQ ID NO:11.
2. The lipoic acid ligase mutant of claim 1 , wherein the mutant further comprises a mutation at the residue corresponding to H149 in SEQ ID NO:11.
3. The lipoic acid ligase mutant of claim 1 , wherein the amino acid sequence is at least 95% identical to SEQ ID NO:11.
4. The lipoic acid ligase mutant of claim 2 , wherein the mutant includes amino acid residue substitutions corresponding to E20A, F147A, and H149G.
5. The lipoic acid ligase mutant of claim 2 , wherein the amino acid sequence of the mutant is otherwise identical to SEQ ID NO:11 except for the E20A, F147A, and H149G substitutions.