Identification of a novel retrovirus in patients with benign prostatic hyperplasia
Methods for producing an immune response to a benign prostatic hyperplasia (BPH) virus are disclosed herein. In several examples, the immune response is a protective immune response. In additional embodiments, methods are disclosed for inhibiting an infection with BPH virus, or treating an infection with BPH virus. Also disclosed are methods for detecting presence of BPH virus in a subject. The methods include detecting the presence of one or more BPH virus polynucleotides or polypeptides in a sample from the subject, or presence of at least one antibody that specifically binds to a BPH virus polypeptide.
1. A method of detecting benign prostatic hyperplasia (BPH) virus in a human subject, comprising:
contacting a sample obtained from the human subject with a detectably labeled probe comprising the nucleic acid sequence of SEQ ID NO: 73 or SEQ ID NO: 74, a forward primer comprising the nucleic acid sequence of SEQ ID NO: 71 and a reverse primer comprising the nucleic acid sequence of SEQ ID NO: 72;
carrying out real-time PCR; and
detecting the detectably labeled probe, thereby detecting presence of BPH virus in the human subject.
2. The method of claim 1 , wherein the sample obtained from the subject comprises prostate tissue.
3. The method of claim 1 , wherein presence of BPH virus in the subject indicates the subject has BPH or is at risk of BPH.
4. The method of claim 3 , further comprising selecting a therapy for the subject.