Technology for preparation of macromolecular microspheres
Microspheres are produced by contacting an aqueous solution of a protein or other macromolecule with an organic solvent and a counterion, and chilling the solution. The microspheres are useful for preparing pharmaceuticals of defined dimensions.
1. A method of making a microparticle composition comprising:
a) adding a counterion selected from the group consisting of sodium citrate, sodium sulfate, calcium sulfate, potassium sulfate and magnesium sulfate to an aqueous solution comprising a polypeptide comprising SEQ ID NO:17, whereby an aqueous composition is formed;
b) adding isopropanol to the aqueous composition of (a) whereby a second aqueous composition is formed; and
c) cooling the second aqueous composition of (b) at a rate of 0.1° C./min to 10° C./min to a temperature between 4° C. and −45° C., whereby a composition containing microparticles comprising a polypeptide compromising SEQ ID NO: 17 is formed.
2. The method of claim 1 wherein the cooling rate is 0.5° C./min to 2° C./min.
3. The method of claim 1 wherein the cooling rate is 0.5° C./min to 1′ C/min.
4. The method of claim 1 wherein the counterion is sodium citrate.
5. The method of claim 1 wherein the counterion is magnesium sulfate.
6. The method of claim 1 further comprising cooling the composition containing microparticles comprising a polypeptide compromising SEQ ID NO: 17 to a temperature of between about −45° C. and about −80° C.
7. The method of claim 1 wherein step c) comprises cooling the second aqueous composition of (b) to a temperature between 2° C. and −20° C. degrees.
8. The method of claim 1 wherein step b) comprises adding isopropanol to about 10% v/v.
9. The method of claim 1 wherein step b) comprises adding isopropanol to about 20% v/v.
10. The method of claim 1 wherein step b) comprises adding isopropanol to about 30% v/v.
11. The method of claim 1 further comprising lyophilizing the composition containing microparticles to obtain a dry powder.