Microbial reductive dehalogenation of vinyl chloride
Compositions and methods are provided that relate to the bioremediation of chlorinated ethenes, particularly the bioremediation of vinyl chloride by Dehalococcoides -like organisms. An isolated strain of bacteria, Dehalococcoides sp. strain VS, that metabolizes vinyl chloride is provided; the genetic sequence of the enzyme responsible for vinyl chloride dehalogenation; methods of assessing the capability of endogenous organisms at an environmental site to metabolize vinyl chloride; and a method of using the strains of the invention for bioremediation.
1. A method for identifying the presence in a sample of a bacterial organism capable of metabolizing chlorinated ethenes to ethene, the method comprising:
determining the presence of a vinyl chloride reductase (vcr) genetic sequence having at least 85% sequence identity to SEQ ID NO:1 in said sample, wherein the presence of said genetic sequence is indicative that a bacterial organism that is present in the sample is capable of metabolizing chlorinated ethenes to ethene.
2. The method according to claim 1 , wherein said sample is obtained from contaminated soil, sediment, sludge, water, or combinations thereof.
3. The method according to claim 1 , wherein said determining comprises the steps of extracting a nucleic acid from the bacterial organism present in said sample, and determining by hybridization the presence of the vcr genetic sequence.
4. The method according to claim 3 , wherein said nucleic acid is amplified prior to said determining.
5. The method according to claim 1 , wherein said vcr genetic sequence has at least 90% sequence identity to SEQ ID NO:1.
6. The method according to claim 1 , wherein said vcr genetic sequence has at least 95% sequence identity to SEQ ID NO:1.