Apparatus for single cell separation and position fixing
View Patent ↗The present invention relates to an apparatus for single cell trap and position fixing of the trapped cell thereof, and specifically, it induces cell movement from where fluid flows strongly to where the fluid flows weakly, by injecting pressed air to an air channel to modify a thin film of a vibrator, and therefore to induce the fluid flow. Further, the present invention relates to an apparatus which can fix the cell position as well as minimizing the external stimulation to the cells, wherein single cells are trapped in a region wherein the fluid flows are minimized and their positions are fixed using the effect that the fluid flows induced by the vibrators are offset one another because the vibrators are formed symmetrically one another.
1. An apparatus for single cell separation and position fixing, which comprises:
a) a first layer containing a fluidic channel wherein fluid containing cells flows;
b) a second layer containing plural air channels wherein air is injected from the outside;
c) a glass substrate wherein the first and the second layers are laminated; and
d) thin film vibrators which are located between the air and fluidic channels and formed at one end of each of the air channels,
wherein the plural thin film vibrators form a square and the plural thin film vibrators are located at each corner of a square area.
2. The apparatus for single cell separation and position fixing of claim 1 , wherein the first layer containing the fluidic channel and the second layer containing air channels are made of PDMS.
3. The apparatus for single cell separation and position fixing of claim 1 , wherein the thin film vibrators are thinner than the surrounding air channels.
4. The apparatus for single cell separation and position fixing of claim 1 , wherein the thin film vibrators vibrate by the pressure of air injected through the air channels of the second layer, and the pressure of the thin film vibrators is transmitted to the fluidic channel of the first layer.
5. The apparatus for single cell separation and position fixing of claim 1 , wherein one of the thin film vibrators is connected to one of the air channel so as to separately control the vibration of the vibrator by the pressure of the air injected through the air channel.
6. The apparatus for single cell separation and position fixing of claim 1 , wherein the center of the square formed by the plural thin film vibrators becomes a cell separation and position fixing region.
7. The apparatus for single cell separation and position fixing of claim 1 , which comprises plural cell separation and position fixing regions formed by the plural thin film vibrators.