Granted Patent B1

US 8,818,734 · App. 13/300,195 · Granted Aug 26, 2014

Inventors: Dagan Wells (Oxford, GB); Jacques Cohen (New York, NY); George Pieczenik (Stockton, NJ)

G01N33/50G01N33/58G01N33/5091

Abstract

Claims (15)

1. A ssDNA binding peptide comprising the sequence of SEQ ID NO:2.

2. The peptide of claim 1 , further comprising an attached chromophore.

3. A viable sperm determining kit comprising Annexin V and a ssDNA and fragmented DNA binding peptide comprising the peptide of SEQ ID NO:2.

4. A method of detecting viable sperm comprising the steps of:

a) staining sperm with chromophore labeled Annexin V and,

b) staining sperm with a different chromophore labeled peptide of SEQ ID 2 and,

c) identifying those sperm not stained which are viable.

5. A method of detecting viable sperm comprising the steps of:

a) staining the sperm with a dye selected from Hoecht, DAPI, and Sytox Green to determine viability, and

b) staining additionally with the peptide of SEQ ID NO:2; and,

c) identifying those viable sperm which do not stain with the peptide of SEQ ID NO:2.

6. A method of intracytoplasmic sperm injection comprising

a) adding a peptide of claim 2 to human sperm and,

b) removing microsurgically a non-stained human sperm; and,

c) inserting said non-stained human sperm into an oocyte.

Assignments (9)