Self-loading microfluidic device and methods of use
Microfluidic devices and methods for conducting chemical assays and biological assays using microfluidic devices are disclosed. The microfluidic devices do not require external connections, tethers, tubing, valves and actuators. The microfluidic devices are useful in methods for analyzing a wide variety of chemical and biological assays such as, for example, molecule-molecule interactions, enzyme-substrate interactions, molecule identification, minimum inhibitory concentrations, therapeutically effective amounts, and toxic amounts.
1. A method of performing an assay, the method comprising:
adding an agent in liquid form to a reaction well of a self-loading microfluidic device, wherein the self-loading microfluidic device further comprises a porous organic polymer and an inlet port, a vacuum well, a main channel, and a side channel;
drying the liquid to form a dried agent;
degassing the self-loading microfluidic device in a low pressure environment;
removing the degassed self-loading microfluidic device from the low pressure environment;
adding a sample solution to the inlet port;
flowing the sample solution from the inlet port to fill the reaction well by degas-driven flow;
incubating the self-loading microfluidic device; and
analyzing the reaction well.
2. The method of claim 1 , wherein the assay is a chemical assay, a biological assay, and combinations thereof.
3. The method of claim 2 , wherein the assay is selected from the group consisting of determining a minimum inhibitory concentration of an antibiotic, determining a therapeutically effective amount of a drug, determining an effect of a drug on a cell, determining an effect of a drug candidate on a cell, identifying an organism, determining a biological profile of an analyte, determining an antibody-antigen interaction, determining an identity of an analyte, amplification of a nucleic acid, and detection of a nucleic acid, and combinations thereof.
4. The method of claim 3 , wherein the amplification is an isothermic amplification assay.
5. The method of claim 3 , wherein the organism is selected from the group consisting of a multicellular organism, a cell, a virus, a bacterium, a fungus, and a spore.