Anti-CD79B antibodies and immunoconjugates and methods of use
The present invention is directed to compositions of matter comprising nucleic acids encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 207 or 208, encoding an anti-CD79b antibody comprising the heavy chain variable domain sequence of SEQ ID NO: 207 and the light chain variable domain sequence of SEQ ID NO: 208, or encoding an anti-CD79b antibody having : (i) a variable light chain comprising a HVR-L1 having the sequence of SEQ ID NO: 194, a HVR-L2 having the sequence of SEQ ID NO: 195, and a HVR-L3 having the sequence of SEQ ID NO: 196 and (ii) a variable light chain comprising a HVR-H1 having the sequence of SEQ ID NO: 202, a HVR-H2 having the sequence of SEQ ID NO: 203, and a HVR-H3 having the sequence of SEQ ID NO: 204, and vectors and host cells thereof.
1. An isolated nucleic acid encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 207.
2. An isolated nucleic acid encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 208.
3. An isolated nucleic acid encoding an anti-CD79b antibody comprising the heavy chain variable domain sequence of SEQ ID NO: 208 and the light chain variable domain sequence of SEQ ID NO: 207.
4. An isolated nucleic acid encoding an anti-CD79b antibody having: (i) a variable light chain comprising a HVR-L1 having the sequence of SEQ ID NO: 194, a HVR-L2 having the sequence of SEQ ID NO: 195, and a HVR-L3 having the sequence of SEQ ID NO: 196 and (ii) a variable light chain comprising a HVR-H1 having the sequence of SEQ ID NO: 202, a HVR-H2 having the sequence of SEQ ID NO: 203, and a HVR-H3 having the sequence of SEQ ID NO: 204.
5. A vector comprising the isolated nucleic acid of claim 1 , 2 , 3 or 4 .
6. A host cell comprising the vector of claim 5 .
7. The host cell of claim 6 , wherein the host cell is eukaryotic.
8. The host cell of claim 7 , wherein the host cell is a CHO cell.
9. A method for making an anti-CD79b antibody or fragment thereof, comprising: (i) culturing the host cell of claim 6 under conditions suitable for expression of the polynucleotide encoding the antibody; and (ii) purifying the antibody expressed therein.