Fluorescent phospholipid ether compounds, compositions, and methods of use

1. A fluorescent phospholipid ether compound of formula:

2. A method of endoscopically diagnosing a malignancy comprising:

administering to a patient the fluorescent phospholipid ether compound of claim 1 ;

using a first technique to produce a visualization of the anatomy of the selected region using the first wavelength of an endoscope;

using a second technique to produce a visualization of the distribution of fluorescence produced by the fluorescent phospholipid ether compound; and

comparing the visualization of the anatomy of the selected region by the first wavelength to the visualization of the distribution of fluorescence by the second wavelength produced by the fluorescent phospholipid ether compound thereby distinguishing a benign tissue from malignant tissue.

3. The method of claim 2 wherein said malignancy is determined in a bodily organ selected from the group consisting of colon, rectum, small bowel, esophagus, stomach, duodenum, uterus, pancreas and common bile duct, bronchi, esophagus, mouth, sinus, lung, bladder, kidney, abdominal cavity, and thoracic cavity.

4. A method of selecting a biopsy tissue in a region suspected of having a malignancy comprising:

administering to a patient a fluorescent phospholipid ether compound according to claim 1 ;

using a first technique to produce a visualization of the anatomy of said region suspected of having said malignancy using the first wavelength of an endoscope;

using a second technique to produce a visualization of the distribution of fluorescence produced by the fluorescent phospholipid ether compound; and

comparing the visualization of the anatomy of said region suspected of having said malignancy by the first wavelength to the visualization of the distribution of fluorescence by the second wavelength produced by the fluorescent phospholipid ether compound thereby distinguishing a benign tissue from malignant tissue and allowing to choose the biopsy tissue.

5. The method of claim 4 wherein said malignancy is determined in a bodily organ selected from the group consisting of colon, rectum, small bowel, esophagus, stomach, duodenum, uterus, pancreas and common bile duct, bronchi, esophagus, mouth, sinus, lung, bladder, kidney, abdominal cavity, and thoracic cavity.

6. A method of diagnosing a skin malignancy comprising:

(a) administering to a patient the fluorescent phospholipid compound according to claim 1 ;

(b) visualizing the anatomy of the skin; and

(c) distinguishing a benign tissue from a malignant tissue,

wherein the malignant tissue displays significantly more fluorescence caused by the fluorescent phospholipid compound.

7. A method of determining malignant tissue margins during a surgical resection of said malignant tissue comprising:

(a) administering to a patient undergoing said surgical resection the fluorescent phospholipid compound according to claim 1 ;

(b) visualizing the malignant tissue; and

(c) determining the margins of said malignant tissue,

wherein the malignant tissue displays significantly more fluorescence caused by the fluorescent phospholipid compound, and wherein the margins of more highly fluorescent region correspond to the margins of said malignant tissue.

8. A method of determining the presence of residual malignant stem cells in a patient undergoing cancer therapy comprising:

(a) administering to a patient undergoing said cancer therapy the fluorescent phospholipid compound according to claim 1 ;

(b) visualizing the tissue that was determined to be malignant prior to said cancer therapy; and

(c) assessing accumulation of the fluorescent phospholipid compound in said tissue,

wherein an accumulation of said fluorescent phospholipid compound in said tissue indicates a possible presence of residual malignant stem cells.

9. A method of determining the presence of residual malignant stem cells in a patient undergoing cancer therapy comprising:

(a) excising a pathological specimen from a patient undergoing said cancer therapy;

(b) incubating said pathological specimen with the fluorescent phospholipid compound according to claim 1 ; and

(c) visualizing the distribution of said fluorescent phospholipid compound in said pathological specimen;

wherein an accumulation of said fluorescent phospholipid compound in said specimen indicates a possible presence of residual malignant stem cells.

10. A method of monitoring response to a tumor therapy comprising:

(a) administering to a patient prior to said tumor therapy the fluorescent phospholipid compound according to claim 1 ;

(b) providing said tumor therapy;

(c) providing the fluorescent phospholipid compound according to claim 5 after the tumor therapy; and

(d) assessing difference in accumulation of the fluorescent phospholipid compound from step (a) and step (c),

wherein a greater accumulation of the phospholipid compound in step (a) versus lesser accumulation in step (c) indicates a positive response to the treatment and/or an effective treatment methodology.