COLLAGEN-BINDING SYNTHETIC PEPTIDOGLYCANS FOR WOUND HEALING
Methods and compositions for promoting wound healing in a patient by administering a collagen-binding synthetic peptidoglycan to the patient are described. Additionally, methods and compositions are described for decreasing scar formation in a patient by administering a collagen-binding synthetic peptidoglycan to the patient.
1 . A method of promoting wound healing in a patient, said method comprising the steps of
administering to the patient a collagen-binding synthetic peptidoglycan, wherein the collagen-binding synthetic peptidoglycan promotes healing of a wound in the patient.
2 . (canceled)
3 . The method of claim 1 wherein the collagen-binding synthetic peptidoglycan is in the form of an engineered collagen matrix wherein the collagen-binding synthetic peptidoglycan is incorporated into the engineered collagen matrix.
4 - 5 . (canceled)
6 . The method of claim 3 wherein the molar ratio of the collagen to the collagen-binding synthetic peptidoglycan is from about 1:1 to about 40:1.
7 - 8 . (canceled)
9 . The method claim 1 wherein the collagen-binding synthetic peptidoglycan has amino acid homology with a portion of the amino acid sequence of a proteoglycan or a protein that regulates collagen fibrillogenesis.
10 . The method of claim 1 wherein the collagen-binding synthetic peptidoglycan has amino acid homology with a portion of a collagen-binding protein that does not regulate collagen fibrillogenesis.
11 . The method of claim 3 wherein the matrix further comprises an exogenous population of cells.
12 . (canceled)
13 . The method of claim 3 wherein the matrix further comprises at least one polysaccharide.
14 . The method of claim 1 wherein the collagen-binding synthetic peptidoglycan is a compound of formula P n G x wherein n is 1 to 30;
wherein x is 1 to 10;
P is a synthetic peptide of about 5 to about 40 amino acids comprising a sequence of a collagen-binding domain; and
G is a glycan.
15 . The method of claim 1 wherein the collagen-binding synthetic peptidoglycan is a compound of formula (P n L) x G wherein n is 1 to 5;
wherein x is 1 to 10;
P is a synthetic peptide of about 5 to about 40 amino acids comprising a sequence of a collagen-binding domain;
L is a linker; and
G is a glycan.
16 . The method of claim 1 wherein the collagen-binding synthetic peptidoglycan is a compound of formula P(LG n ) x wherein n is 1 to 5;
x is 1 to 10;
P is a synthetic peptide of about 5 to about 40 amino acids comprising a sequence of a collagen-binding domain;
L is a linker; and
G is a glycan.
17 . The method of claim 1 wherein the glycan is a glycosaminoglycan or a polysaccharide.
18 - 20 . (canceled)
21 . The method of claim 1 wherein the glycan is dermatan sulfate.
22 . The method of claim 1 wherein the peptide comprises the amino acid sequence RRANAALKAGELYKSILYGC.
23 - 27 . (canceled)
28 . A method of decreasing scar formation in a patient, said method comprising the steps of
administering to the patient a collagen-binding synthetic peptidoglycan, wherein the collagen-binding synthetic peptidoglycan decreases scar formation in the patient.
29 . (canceled)
30 . The method of claim 28 wherein the collagen-binding synthetic peptidoglycan is in the form of an engineered collagen matrix wherein the collagen-binding synthetic peptidoglycan is incorporated into the engineered collagen matrix.
31 - 32 . (canceled)
33 . The method of claim 30 wherein the molar ratio of the collagen to the collagen-binding synthetic peptidoglycan is from about 1:1 to about 40:1.
34 - 35 . (canceled)
36 . The method of claim 28 wherein the collagen-binding synthetic peptidoglycan has amino acid homology with a portion of the amino acid sequence of a proteoglycan or a protein that regulates collagen fibrillogenesis.
37 . The method of claim 28 wherein the collagen-binding synthetic peptidoglycan has amino acid homology with a portion of a collagen-binding protein that does not regulate collagen fibrillogenesis.
38 . The method of claim 30 wherein the matrix further comprises an exogenous population of cells.
39 . (canceled)
40 . The method of claim 30 wherein the matrix further comprises at least one polysaccharide.
41 . The method of claim 28 wherein the collagen-binding synthetic peptidoglycan is a compound of formula P n G x wherein n is 1 to 30;
wherein x is 1 to 10;
P is a synthetic peptide of about 5 to about 40 amino acids comprising a sequence of a collagen-binding domain; and
G is a glycan.
42 . The method of claim 28 wherein the collagen-binding synthetic peptidoglycan is a compound of formula (P n L) x G wherein n is 1 to 5;
wherein x is 1 to 10;
P is a synthetic peptide of about 5 to about 40 amino acids comprising a sequence of a collagen-binding domain;
L is a linker; and
G is a glycan.
43 . The method of claim 28 wherein the collagen-binding synthetic peptidoglycan is a compound of formula P(LG n ) x wherein n is 1 to 5;
x is 1 to 10;
P is a synthetic peptide of about 5 to about 40 amino acids comprising a sequence of a collagen-binding domain;
L is a linker; and
G is a glycan.
44 . The method of claim 28 wherein the glycan is a glycosaminoglycan or a polysaccharide.
45 - 47 . (canceled)
48 . The method of claim 28 wherein the glycan is dermatan sulfate.
49 . The method of claim 28 wherein the peptide comprises the amino acid sequence RRANAALKAGELYKSILYGC.
50 - 54 . (canceled)