Centrifugal separation kit and methods for centrifugal separation using the same

1. A centrifugal separation kit installed to a centrifugal separator to divide blood into blood cell components composed of a Red Blood Cell (RBC) layer and blood plasma components composed of a Platelet Rich Plasma (PRP) layer and a PPP (Platelet Poor Plasma) layer in order from a bottom thereof, comprising:

a) an injector having an opening at an upper portion thereof so that a piston having a rubber packing installed thereto is movably inserted into the upper portion through the opening, the injector having a first predetermined volumetric capacity corresponding to a known volume of whole blood (Wb) and an insert portion at a lower portion thereof to which an injector needle is installed thereto; and

b) a centrifugal separation tube loadable on the centrifugal separator with the injector installed thereto, comprising:

a lower portion having a second predetermined volumetric capacity,

a collecting unit having a collecting channel of an elongated shape at a center thereof, wherein the lower portion of the centrifugal separation tube is connected with a lower end of the collecting channel and the insert portion of the injector is frictionally engaged into an upper end of the collecting channel, thereby the collecting channel providing a direct fluid communication between the lower portion and an internal space of the injector while the blood is being centrifugally separated,

wherein the centrifugal separation tube is configured for including a predetermined amount of cell isolation gel having a volume of equal or greater than 0, and

wherein a lower side of the collecting unit has an adjusted location so that the second predetermined volumetric capacity is substantially equal to the sum of the volume of the cell isolation gel and a predetermined fraction of the first predetermined volumetric capacity, wherein the predetermined fraction corresponds to a known volume of RBCs (Vb) present in the known volume of whole blood (Wb).

2. The centrifugal separation kit according to claim 1 ,

wherein the centrifugal separation tube has an inner diameter equal to or greater than an outer diameter of the injector, and

wherein an outer surface of the injector is frictionally engaged to an inner surface of the centrifugal separation tube.

3. The centrifugal separation kit according to claim 1 , wherein an upper portion of the collecting unit is shaped corresponding to a contact surface of the injector so that the insert portion of the injector is inserted into and frictionally engaged to the collecting channel and wherein the collecting channel has a length greater than a length of the injector insert portion.

4. The centrifugal separation kit according to claim 1 , wherein the second predetermined volumetric capacity (A) of the lower portion of the centrifugal separation tube, a volume (Wb) of the blood corresponding to the first predetermined volumetric capacity, a volume (Vb) of the Red Blood Cell (RBC) layer, a volume ratio (α) of the Red Blood Cell (RBC) layer based on the volume of (Wb) of the blood, and a volume (Vf) of the cell isolation gel satisfy the following relation:

A =(α× Wb )+ Vf=Vb+Vf ; and

0≦ Vf≦Wb.

5. The centrifugal separation kit according to claim 1 ,

wherein a ventilation hole is formed in the collecting unit, and

wherein the ventilation hole is closed as the injector is installed and frictionally engaged to the collecting unit.

6. The centrifugal separation kit according to claim 1 , wherein inclined protrusions are formed at regular intervals on an inner side of the centrifugal separation tube along a length direction of the centrifugal separation tube, and the collecting unit moves downwards as much as the interval of the protrusions due to a pressing force applied from the injector so as not to move upwards.

7. The centrifugal separation kit according to claim 6 , wherein the centrifugal separation tube is made of transparent material, and gradations representing a volume of the space located in the interval of the protrusions are marked on an outer circumference of the centrifugal separation tube so that the location of the collecting unit is adjusted according to the sum of a volume of the Red Blood Cell (RBC) layer in the collected whole blood and a volume of the filled cell isolation gel.