IP Library Granted Patent US 10,119,129
Granted Patent B2
US 10,119,129 · App. 13/318,850 · Granted Nov 6, 2018

Enzyme complex from

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Quick Facts
Patent No.
US 10,119,129
App. No.
13/318,850
Granted
Nov 6, 2018
Kind
B2
Abstract

The invention relates to an improved enzyme complex having a plurality of enzyme activities of an expression product obtained by fermentation of the genus Trichoderma in combination with one or more enzymes of a different fungus strain.

Claims (19)

1. An enzyme complex having beta-1,4-endoglucan hydrolase activity comprising:

a. a fermentation product of Trichoderma reesei wherein at least 61 % of the beta-1,4-endoglucan hydrolase activity of the enzyme complex, as measured by the “Assay 1” method, is from the fermentation product of Trichoderma reesei; and

b. one or more enzymes derived from Penicillium funiculosum selected from a xylanase (EC 3.2.1.8), a cellulase (EC 3.2.1.4), and a beta-glucanase (EC 3.2.1.6), wherein 30% to 39% of the beta-1,4-endoglucan hydrolase activity of the enzyme complex, as measured by the “Assay 1” method, is from a fermentation product of Penicillium funiculosum; wherein the enzyme complex is substantially cell free.

2. The enzyme complex according to claim 1 , wherein said one or more enzymes derived from Penicillium funiculosum is an expression product obtained by fermentation of said Penicillium funiculosum.

3. The enzyme complex according to claim 2 , which expression product obtained by fermentation of said Penicillium funiculosum comprises a xylanase.

4. The enzyme complex according to claim 1 , further comprising one or more enzyme activities selected from the list consisting of endo-1,4-β-xylanase, endo-1,3(4)-β-glucanase, cellulase, laminarinase, endo-1,5-α-L arabinanase, beta-D-glucoside glucohydrolase, β-Xylosidase, cellobiohydrolase, glucan 1,4-beta-glucosidase, xyloglucan-specific exo-beta-1,4-glucanase and α-N Arabinofuranosidase.

5. An enzyme complex having beta-1,4-endoglucan hydrolase activity comprising:

a. a first expression product obtained by fermentation of Trichoderma reesei , wherein the first expression product accounts for at least 61% of the beta-1,4-endoglucan hydrolase activity of the enzyme complex; and

b. a second expression product obtained by fermentation of Penicillium funiculosum, wherein the second expression product accounts for at least 39% of the beta-1,4-endoglucan hydrolase activity of the enzyme complex;

wherein the percentages of beta-1,4-endoglucan hydrolase activity are measured by the “Assay 1” method; wherein the enzyme complex is substantially cell free.

6. The enzyme complex according to claim 1 , wherein said strain is Trichoderma reesei deposited under the Budapest treaty in the American Type Culture Collection (ATCC) having a strain designation GC Cellulose A83 GICC 0004, M03000004 deposited by Danisco A/S on the date of 5 May 2009, or a derivative or progeny thereof.

7. The enzyme complex according to claim 2 , wherein said expression product obtained by fermentation of a Penicillium fungus is from single culture of the species Penicillium funiculosum.

8. The enzyme complex according to claim 7 , wherein said strain is Penicillium funiculosum deposited under the Budapest treaty in the International Mycological Institute under the number IMI 378536, or a derivative or progeny thereof.

9. The enzyme complex according to claim 1 , wherein said expression product obtained by fermentation is from a wild type species.

10. The enzyme complex according to claim 1 , having an enzyme activity of at least about 3000 U/g, such as at least about 4000 U/g, such as at least about 5000 U/g, such as at least about 6000 U/g, such as at least about 7000 U/g as measured by “Assay 1” as described herein derived from fermentation of Trichoderma reesei.

11. The enzyme complex according to claim 1 , having a total enzyme activity of at least about 4000 U/g as measured by “Assay 1”as described herein.

12. The enzyme complex according to claim 1 , wherein about 2362 U/g beta-1,4-endoglucan hydrolase activity is from a fermentation product of Penicillium funiculosum and wherein about 5315 u/g U/g beta-1,4-endoglucan hydrolase activity is from a fermentation product of Trichoderma reesei wherein said units/g (U/g) is determined by “Assay1”.

13. The enzyme complex according to claim 1 , wherein said enzyme complex contains at least 5 different side activities.

14. The enzyme complex of claim 1 or claim 5 further comprising at least one preservative.

Assignments (2)
CHANGE OF NAME Recorded Feb 6, 2024
From: DUPONT NUTRITION BIOSCIENCES APS
To: INTERNATIONAL N&H DENMARK APS
Reel/Frame 066494/0814 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 19, 2015
From: FISH, NEVILLE MARSHALL; MILLER, LONE BROND
To: DUPONT NUTRITION BIOSCIENCES APS
Reel/Frame 034985/0121 →