Test agent for visceral obesity and use thereof
Disclosed are: a method for detecting (diagnosing) visceral obesity in a subject; a test agent useful for the method; a method for searching for a substance that can be used as an active ingredient for ameliorating visceral obesity; and an ameliorating agent for visceral obesity or a medicinal agent for preventing a metabolic disease developed as a result of the progression of visceral obesity. As the test agent, a polynucleotide which comprises at least 15 nucleotides and can hybridize with a nucleotide sequence for coiled-coil domain containing protein 3 (CCDC3) gene or a nucleotide sequence complementary to the nucleotide sequence under stringent conditions or an antibody capable of recognizing CCDC3 protein is used.
1. A method for detecting visceral obesity using, as an index, the amount of mRNA expression of coiled-coil domain containing protein 3 (CCDC3) or the amount of the product CCDC3 protein in a test sample collected from a subject, comprising the steps of:
(1) measuring the amount of CCDC3 mRNA expression or the amount of the product CCDC3 protein in the test sample collected from the subject;
(2) comparing the measured CCDC3 mRNA expression amount (subject expression amount) or the measured amount of the product CCDC3 protein (subject production amount) with the CCDC3 mRNA expression amount (control expression amount) or the amount of the product CCDC3 protein (control production amount) in a control sample derived from a non-visceral obesity individual; and
(3) determining visceral obesity in the subject by using a higher subject expression amount or a higher subject production amount than the control expression amount or the control production amount as an index.
2. The method according to claim 1 , wherein the method uses an antibody that recognizes coiled-coil domain containing protein 3 (CCDC3) protein, and is selected from the group of the following antibodies (1) to (3):
(1) an antibody having amino acid sequences of SEQ ID NOS:31, 32, and 33 for the CDR1, CDR2, and CDR3, respectively, of the heavy-chain variable domain, and amino acid sequences of SEQ ID NOS:35, 36, and 37 for the CDR1, CDR2, and CDR3, respectively, of the light-chain variable domain;
(2) an antibody having amino acid sequences of SEQ ID NOS:39, 40, and 41 for the CDR1, CDR2, and CDR3, respectively, of the heavy-chain variable domain, and amino acid sequences of SEQ ID NOS:43, 44, and 45 for the CDR1, CDR2, and CDR3, respectively, of the light-chain variable domain; and
(3) an antibody having amino acid sequences of SEQ ID NOS:47, 48, and 49 for the CDR1, CDR2, and CDR3, respectively, of the heavy-chain variable domain, and amino acid sequences of SEQ ID NOS:51, 52, and 53 for the CDR1, CDR2, and CDR3, respectively, of the light-chain variable domain.