Amyloid β fibrillogenesis-inhibiting peptide
The present invention provides a peptide which functions as a mimic peptide of an amyloid β peptide and is capable of inhibiting the fibrillogenesis of an amyloid β peptide. The present invention relates to an 8- to 30-amino acid residue peptide comprising an amino acid sequence represented by the following formula (I): X 1 -Asp-X 2 -X 3 -X 4 -Pro-X 5 -X 6 (SEQ ID NO: 28) (I), wherein X 1 represents a branched chain amino acid, and X 2 , X 3 , X 4 , X 5 , and X 6 are the same or different and each represents an α-amino acid, and to a pharmaceutical composition and an amyloid β fibrillogenesis inhibitor comprising the peptide.
1. A 12- to 30-amino acid residue peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, and SEQ ID NO: 15.
2. The peptide according to claim 1 , wherein the peptide is a 23- to 30-amino acid residue peptide comprising a TAT sequence represented by the following formula:
(SEQ ID NO: 30)
Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg.
3. A composition comprising the peptide according to claim 1 and a carrier or excipient.
4. The peptide according to claim 1 , wherein the peptide comprises the amino acid sequence of SEQ ID NO: 9.
5. The peptide according to claim 1 , wherein the peptide comprises the amino acid sequence of SEQ ID NO: 10.
6. The peptide according to claim 1 , wherein the peptide comprises the amino acid sequence of SEQ ID NO: 11.
7. The peptide according to claim 1 , wherein the peptide comprises the amino acid sequence of SEQ ID NO: 12.
8. The peptide according to claim 1 , wherein the peptide comprises the amino acid sequence of SEQ ID NO: 13.
9. The peptide according to claim 1 , wherein the peptide comprises the amino acid sequence of SEQ ID NO: 14.
10. The peptide according to claim 1 , wherein the peptide comprises the amino acid sequence of SEQ ID NO: 15.
11. The peptide according to claim 2 , wherein the peptide comprises the amino acid sequence of SEQ ID NO: 9.
12. The peptide according to claim 2 , wherein the peptide comprises the amino acid sequence of SEQ ID NO: 10.
13. The peptide according to claim 2 , wherein the peptide comprises the amino acid sequence of SEQ ID NO: 11.
14. The peptide according to claim 2 , wherein the peptide comprises the amino acid sequence of SEQ ID NO: 12.
15. The peptide according to claim 2 , wherein the peptide comprises the amino acid sequence of SEQ ID NO: 13.
16. The peptide according to claim 2 , wherein the peptide comprises the amino acid sequence of SEQ ID NO: 14.
17. The peptide according to claim 2 , wherein the peptide comprises the amino acid sequence of SEQ ID NO: 15.
18. A peptide selected from the group consisting of SEQ ID No: 24 and SEQ ID No: 25.
19. A method for inhibiting amyloid β 1-42 fibrillogenesis comprising administering an effective amount of the peptide according to claim 18 to a subject in need thereof.