HLA homozygous cells
The present invention provides methods of generating a mammalian cell that is homozygous at a locus of interest, as well as cells made by the method. The present invention further provides methods of using the cells.
1. An isolated human stem cell or a progeny thereof, wherein the stem cell or progeny thereof is homozygous for a human leukocyte antigen (HLA) haplotype, the HLA haplotype comprising an entire HLA locus; and wherein at least about 90% of the non-HLA loci in the cell or progeny thereof are heterozygous and the cell is not a parthenogenic stem cell.
2. The isolated human stem cell or progeny thereof of claim 1 , wherein said cell is selected from an embryonic stem cell, a neural stem cell, a mesenchymal stem cell, a hematopoietic stem cell, and a differentiated cell.
3. The isolated human stem cell or progeny thereof of claim 1 , wherein the HLA haplotype comprises alleles found in a common HLA haplotype present in a selected human population.
4. A differentiated progeny cell of the isolated human stem cell of claim 1 , wherein the cell is selected from a neural cell, a glial cell, a pancreatic islet cell, a cardiomyocyte, a mesenchymal lineage cell, and a hematopoietic stem cell lineage cell.
5. A composition comprising the isolated human stem cell or progeny thereof of claim 1 .
6. A composition comprising the differentiated progeny cell of claim 4 .
7. The composition of claim 6 , wherein the differentiated progeny cell is associated with a matrix.
8. The composition of claim 6 , wherein the differentiated progeny cell is present in a tissue sheet.
9. The isolated human stem cell or progeny thereof of claim 1 , wherein the stem cell or progeny thereof is homozygous for all non-HLA loci present on the short-arm of chromosome 6 that are distal to both the chromosome's centromere and said entire HLA locus.