Altering lignin and wood density
In angiosperm and gymnosperm plants, overexpressing a SAMdc nucleotide sequence can decrease lignin content and, for plants with woody tissue, increase wood density.
1. Wood pulp comprising a recombinant nucleic acid encoding a SAMdc enzyme and, operably linked thereto, a xylem-preferred promoter.
2. The wood pulp of claim 1 , wherein said wood pulp is wood pulp of a woody tree.
3. The wood pulp of claim 2 , wherein said woody tree is a Eucalyptus species, a Populus species, or a Conifer.
4. The wood pulp of claim 3 , wherein said Conifer is a Pinus species.
5. The wood pulp of claim 1 , wherein said wood pulp has reduced lignin levels compared with wood pulp of a control plant.
6. The wood pulp of claim 1 , wherein said wood pulp has an increased ratio of syringyl lignin to guaiacyl lignin compared with wood pulp of a control plant.
7. The wood pulp of claim 1 , wherein said nucleic acid is selected from the group consisting of:
(a) a nucleotide sequence set forth in any of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 24, and 26;
(b) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in any of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, and 18;
(c) a nucleotide sequence that differs from the nucleotide sequences of (a) or (b) due to degeneracy of the genetic code and encodes a polypeptide with SAMdc enzyme activity.
8. The wood pulp of claim 1 , wherein said xylem-preferred promoter is selected from the group consisting of TUB gene promoter, LTP gene promoter, 4CL gene promoter, and C4H gene promoter.
9. A method of making wood pulp, comprising producing wood pulp from a transgenic plant comprising a recombinant nucleic acid encoding a SAMdc enzyme and, operably linked thereto, a xylem-preferred promoter.
10. The method of claim 9 , wherein said wood pulp is wood pulp of a woody tree.
11. The method of claim 10 , wherein said woody tree is a Eucalyptus species, a Populus species, or a Conifer.
12. The method of claim 11 , wherein said Conifer is a Pinus species.
13. The method of claim 9 , wherein said wood pulp has reduced lignin levels compared with wood pulp of a control plant.
14. The method of claim 9 , wherein said wood pulp has an increased ratio of syringyl lignin to guaiacyl lignin compared with wood pulp of a control plant.
15. The method of claim 9 , wherein said nucleic acid is selected from the group consisting of:
(a) a nucleotide sequence set forth in any of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 24, and 26;
(b) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in any of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, and 18;
(c) a nucleotide sequence that differs from the nucleotide sequences of (a) or (b) due to degeneracy of the genetic code and encodes a polypeptide with SAMdc enzyme activity.
16. The method of claim 9 , wherein said xylem-preferred promoter is selected from the group consisting of TUB gene promoter, LTP gene promoter, 4CL gene promoter, and C4H gene promoter.
17. The method of claim 9 , wherein said transgenic plant has increased wood density and decreased lignin content relative to a control plant.
18. A method of increasing pulp and cellulose fiber yields during pulp and paper processing, comprising processing wood pulp comprising a recombinant nucleic acid encoding a SAMdc enzyme and, operably linked thereto, a xylem-preferred promoter.
19. The method of claim 18 , wherein said wood pulp has reduced lignin levels compared with wood pulp of a control plant.
20. The method of claim 18 , wherein said wood pulp has an increased ratio of syringyl lignin to guaiacyl lignin compared with wood pulp of a control plant.