Simian adenovirus nucleic acid and amino acid sequences, vectors containing same, and methods of use
A recombinant vector comprises simian adenovirus sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.
1. A recombinant adenovirus having an adenovirus capsid comprising an AdPan5 hexon protein having the amino acid sequence of SEQ ID NO:3, said adenovirus having packaged therein a nucleic acid molecule comprising (a) an adenovirus 5′ inverted terminal repeat sequence, (b) a nucleic acid sequence heterologous to AdPan5 encoding a therapeutic or an immunogenic product operably linked to expression control sequences which direct transcription, translation, and/or expression of said product in a cell, and (c) an adenovirus 3′ inverted terminal repeat sequence, wherein the heterologous nucleic acid sequence defined in (b) is located between the 5′ inverted terminal repeat sequence defined in (a) and the 3′ inverted terminal repeat sequence defined in (c).
2. The recombinant adenovirus according to claim 1 , wherein the capsid further comprises an AdPan5 fiber protein.
3. The recombinant adenovirus according to claim 2 , wherein said recombinant adenovirus vector is a pseudotyped adenovirus wherein the adenovirus 5′ inverted terminal repeat and the adenovirus 3′ inverted terminal repeat are heterologous to AdPan5.
4. The recombinant adenovirus according to claim 1 , wherein the capsid further comprises an AdPan5 penton protein.
5. The recombinant adenovirus according to claim 1 , wherein the adenovirus further comprises an adenovirus capsid protein sequence heterologous to the AdPan5 capsid protein sequence.
6. The recombinant adenovirus according to 1 , wherein the nucleic acid molecule further comprises adenoviral genomic sequences which comprises a deletion in the E1 region of the adenovirus genes.
7. A composition comprising the adenovirus according to claim 1 and a pharmaceutically acceptable carrier.
8. The recombinant adenovirus according to claim 1 , wherein the adenovirus capsid comprises the AdPan5 penton having amino acid sequence of SEQ ID NO: 2 and the AdPan5 fiber protein having the amino acid sequence of SEQ ID NO:4.
9. A recombinant adenovirus having an adenovirus capsid comprising an AdPan5 hexon protein having the amino acid sequence of SEQ ID NO:3, an AdPan5 penton protein, and an AdPan5 fiber protein, said adenovirus having packaged therein a nucleic acid molecule comprising (a) an adenovirus 5′ inverted terminal repeat sequence, (b) a nucleic acid sequence heterologous to AdPan5 encoding a therapeutic or an immunogenic product operably linked to expression control sequences which direct transcription, translation, and/or expression thereof in a host cell, and (c) an adenovirus 3′ inverted terminal repeat sequence, wherein the heterologous nucleic acid sequence defined in (b) is located between the 5′ inverted terminal repeat sequence defined in (a) and the 3′ inverted terminal repeat sequence defined in (c).