Nucleic acid sample preparation methods and compositions
The present invention provides compositions and methods for preparing a nucleic acid library in a multi-purpose buffer (e.g., employing whole genome amplification), where nucleic acid purification is not required between or during steps. In certain embodiments, small amounts of starting nucleic acid (e.g., genomic DNA) are employed and the steps are accomplished in a single container. In some embodiments, the nucleic acid library is subjected to sequencing methodologies or rolling circle amplification.
1. A method of preparing a nucleic acid library in a multi-purpose buffer, comprising:
a) combining a nucleic acid sample with a multi-purpose buffer, wherein said multi-purpose buffer comprises deoxyribonucleotide triphosphates (dNTPs) and primers to generate a mixture comprising said nucleic acid sample and said multi-purpose buffer;
b) contacting said mixture comprising said nucleic acid sample and said multi-purpose buffer with a plurality of purified enzymes, wherein said plurality of purified enzymes comprise a first enzyme with polymerase activity, a second enzyme with kinase activity, and a third enzyme with phosphatase activity, and wherein said contacting is under conditions such that amplified nucleic acid is generated in said multipurpose buffer;
c) mechanically, physically, chemically or enzymatically treating said amplified nucleic acid in said multi-purpose buffer such that sheared amplified nucleic acid is generated in said multi-purpose buffer without purification of said sheared amplified nucleic acid;
d) treating said multi-purpose buffer comprising said sheared amplified nucleic acid to inactivate said polymerase activity, said kinase activity, and said phosphatase activity without purification of said sheared amplified nucleic acid; and
e) contacting said multi-purpose buffer comprising said sheared amplified nucleic acid and said inactive polymerase, kinase and phosphatase with a ligase and nucleic acid adapters without purification of said sheared amplified nucleic acid under conditions such that an adapter-linked nucleic acid library is generated.
2. The method of claim 1 , wherein said nucleic acid sample comprises genomic DNA, and wherein the amplified nucleic acid is generated by whole genome amplification.
3. The method of claim 1 , wherein said steps a)-e) are conducted in a single container.
4. The method of claim 1 , further comprising contacting said multi-purpose buffer containing said adapter-linked nucleic acid library with a proteinase and phosphatase, resin or column such that proteins and dNTPs are removed from said multi-purpose buffer.
5. The method of claim 1 , wherein said multi-purpose buffer further comprises an emulsifier.
6. The method of claim 1 , wherein said multi-purpose buffer further comprises tris(hydroxymethyl)aminomethane (TRIS).
7. The method of claim 1 , wherein said multi-purpose buffer further comprises a divalent metal cation.
8. The method of claim 1 , wherein said multi-purpose buffer further comprises an inorganic salt.
9. The method of claim 1 , wherein said multi-purpose buffer further comprises polyadenylic acid.
10. The method of claim 1 , wherein said multi-purpose buffer further comprises an alpha-linked disaccharide.
11. The method of claim 1 , wherein said multi-purpose buffer further comprises a reducing agent.
12. The method of claim 1 , wherein said multi-purpose buffer further comprises albumin or albumin-like protein.
13. The method of claim 1 , wherein said nucleic acid sample is an amount that is between 10 pg and 50 ng.