Photoreactive regulator of protein function and methods of use thereof
View Patent ↗The present invention provides a synthetic regulator of protein function, which regulator is a light-sensitive regulator. The present invention further provides a light-regulated polypeptide that includes a subject synthetic regulator. Also provided are cells and membranes comprising a subject light-regulated polypeptide. The present invention further provides methods of modulating protein function, involving use of light. The present invention further provides methods of identifying agents that modulate protein function.
1. A cell comprising a light-regulated polypeptide, wherein the light-regulated polypeptide comprises an ionotropic glutamate receptor (iGluR) and a synthetic regulator of said iGluR, wherein the synthetic regulator has the structure:
wherein R 1 is a binding moiety that provides for covalent linkage to the iGluR, wherein R 1 is selected from the group consisting of a maleimide, an acrylic ester, an acrylic amide, acrylamide, an α-haloacetamide, an epoxide, a succinimidyl ester, a disulfide, vinylsulfone, and a methanethiosulfonate; and R 2 has the structure:
wherein n is 0, 1 or 2; m is 1 or 3; and R″ is a ligand of the iGluR, and wherein the synthetic regulator is stably associated with the iGluR at or near a ligand binding site of the iGluR.
2. The cell of claim 1 , wherein cell is a mammalian cell.
3. The cell of claim 2 , wherein the mammalian cell is a cell line.
4. The cell of claim 3 , wherein the mammalian cell is selected from a 293 cell, a 3T3 cell, a COS cell, and a PC12 cell.
5. The cell of claim 1 , wherein R 1 is selected from —NHCOCH 2 -maleimide, —NHCOCH═CH 2 , and —NHCOCH 2 —X wherein X is a halogen; and
R″ is a glutamate.
6. The cell of claim 5 , wherein R″ is a glutamate.
7. The cell of claim 1 , wherein the synthetic regulator has the structure:
8. The cell of claim 1 , wherein the synthetic regulator has the structure:
9. A method of modulating ligand binding to a ligand binding site of the light-regulated polypeptide present in the cell of claim 1 , the method comprising changing the wavelength of light and/or the intensity of light to which the light-regulated polypeptide in the cell is exposed.
10. The method of claim 9 , wherein said change in wavelength results in an increased probability of binding of the ligand to the ligand-binding site of the light-regulated polypeptide.