Transformation of algal cells
Exemplary methods include a method for transforming an algal cell by preparing a transformation construct, preparing a particle for bombarding the algal cell, adhering the transformation construct to the particle, bombarding the algal cell with the particle, and growing the algal cell into a colony. The transformation construct is replicated within a nuclear genome of the algal cell and the growing of the algal cell is in a nutrient medium. Another exemplary method may include a method for genetically modifying an algal cell, by adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity, introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell, and selecting a colony that includes the transformed algal cell.
1. A method for genetically modifying an algal cell, the method comprising:
adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity, the nucleic acid including a gene involved in lipid biosynthesis;
introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell; and
selecting a colony that includes the transformed algal cell;
wherein the electrical pulse is applied with a field strength of higher than approximately 7500 V/cm; and
wherein the algal cell is of genus Nannochloropsis.
2. The method of claim 1 , wherein the nucleic acid includes a transformation construct.
3. The method of claim 1 , wherein the electrical pulse is applied with a field strength of higher than approximately 9000 V/cm.
4. The method of claim 1 , wherein the electrical pulse is applied with a field strength of higher than approximately 10,000 V/cm.
5. The method of claim 1 , wherein the electrical pulse is applied with a shunt resistor of greater than approximately 100 OHM.