Acoustically sensitive drug delivery particles comprising non-lamellar forming phosphatidylethanolamine
Novel acoustically sensitive drug carrying particles comprising non-lamellar forming lipids are disclosed, as well as uses and methods thereof. The drug carrying particles accumulate in the diseased target tissue and efficiently release their pay-load upon exposure to acoustic energy.
1. A sonosensitive liposome, comprising
at least 10 mol % of an unsaturated phosphatidylethanolamine (PE); and a polyethyleneglycol or a derivative thereof,
said liposome not comprising one or more of air bubbles, nondissolved gases, cholesterolhemisuccinate (CHEMS), or free fatty acids, and said liposome not comprising lysolipids,
wherein the PE comprises an acyl chain of at least 18 carbon atoms or longer, wherein the head group of the PE is not conjugated to the polyethyleneglycol, and
wherein the sonosensitive liposome is unilamellar.
2. The liposome of claim 1 , wherein the PE comprises two unsaturated acyl chains.
3. The liposome of claim 1 , wherein said liposome comprises at least 47 mol % of the PE.
4. The liposome of claim 1 , wherein the PE is DOPE and/or SOPE.
5. The liposome of claim 1 , wherein the PE is DOPE.
6. The liposome of claim 1 , comprising 8 mol % or more polyethyleneglycol or a derivative thereof.
7. The liposome of claim 1 , further comprising cholesterol.
8. The liposome of claim 7 , wherein said liposome comprises 20 mol % or more cholesterol.
9. The liposome of claim 1 , further comprising a drug.
10. A method of treating a condition or disease, comprising
administering the liposome of claim 1 to a patient in need thereof, wherein said liposome comprises a medicament, and wherein said medicament is activated or released by acoustic energy.
11. The method of claim 10 , wherein the condition or disease is cancer, an immune disorder, an infection, or an inflammatory disease.
12. A method for manufacturing the liposome of claim 1 , which comprises:
producing a thin film comprising PE and PEP, wherein the film does not comprise membrane embedded alcohols,
hydrating the film,
exposing the film to repeated freeze-thaw cycles, and
extruding the film into liposomes.
13. A pharmaceutical composition comprising the liposome of claim 1 .
14. The liposome of claim 9 , wherein the drug is a siRNA, doxorubicin, filgrastim, pegfilgrastim, or sargramostim.
15. The liposome of claim 1 , wherein the liposome comprises at least 20 mol % of the PE.
16. The liposome of claim 1 , wherein the liposome comprises at least 30 mol % of the PE.
17. The liposome of claim 1 , wherein the liposome comprises at least 58 mol % of the PE.
18. The liposome of claim 1 , wherein the liposome has a size of less than 1000 nm.
19. The liposome of claim 1 , wherein the liposome has a size of less than 500 nm.
20. The liposome of claim 1 , wherein the liposome has a size of less than 200 nm.
21. The liposome of claim 1 , wherein the PE is selected from one or more of the group consisting of: 1-stearoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (SOPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1,2-Dioleoyl-sn-Glycero-3-Phosphoethanolamine (DOPE), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE), and combinations thereof.