Efficient isolation of cimiracemate A, and methods of use
View Patent ↗A method for isolating cimiracemate A from a Cimicifuga species is disclosed, comprising the steps of a) providing a sufficient quantity of raw materials from the Cimicifuga species, b) mixing the raw materials from the Cimicifuga species with an aqueous polar solvent at a temperature of about 20° C. to about 28° C. to obtain a solvent extract comprising cimiracemate A, and c) isolating cimiracemate A from the solvent extract.
1. A method for isolating cimiracemate A from a Cimicifuga species, comprising the steps of:
a) providing a sufficient quantity of raw material of a Cimicifuga species;
b) mixing the raw material of a Cimicifuga species with a solvent at a temperature of 20° C. to 28° C. to obtain a solvent extract comprising cimiracemate A, wherein the solvent is water-ethanol comprising ethanol at a concentration of less than 20%; and
c) isolating cimiracemate A from the solvent extract to yield a liquid or dried composition comprising cimiracemate A.
2. The method of claim 1 , wherein cimiracemate A is isolated from the solvent extract using high-performance liquid chromatography (HPLC).
3. The method of claim 2 , wherein cimiracemate A is eluted using HPLC from the solvent extract at UV absorbance of about 210 nm.
4. The method of claim 3 , wherein cimiracemate A is eluted using HPLC from the solvent extract at about 23° C.
5. The method of claim 1 , wherein the raw material of a Cimicifuga species is ground into powder.
6. The method of claim 1 , wherein the Cimicifuga species is selected from the group consisting of Cimicifuga racemosa, Cimicifuga foetida , and Cimicifuga heracleifolia.
7. The method of claim 6 , wherein the Cimicifuga species is Cimicifuga racemosa .
8. The method of claim 1 claim 1 , wherein the Cimicifuga species is mixed with water-ethanol at a ratio of about 1:15 to about 1:20 (w/v).
9. A method for isolating cimiracemate A from a Cimicifuga species, consisting of the steps of:
a) providing a sufficient quantity of raw material of a Cimicifuga species;
b) mixing the raw material of a Cimicifuga species with a solvent at a temperature of 20° C. to 28° C. to obtain a solvent extract comprising cimiracemate A, wherein the solvent is water or water-ethanol comprising ethanol at a concentration of less than 20%; and
c) isolating cimiracemate A from the solvent extract to yield a liquid composition comprising cimiracemate A.
10. The method of claim 9 , wherein the solvent is water.
11. The method of claim 10 , wherein the Cimicifuga species is mixed with water at a ratio of about 1:15 to about 1:20 (w/v).
12. A method for preparing a composition comprising the isolated cimiracemate A from claim 1 , further comprising formulating the isolated cimiracemate A into a composition selected from the group consisting of tablets, capsules, powders, pastilles, suppositories, oral solutions, suspensions, and injectable compositions.
13. The method of claim 12 , comprising formulating the isolated cimiracemate A into a composition selected from the group consisting of tablets, capsules, powder, and pastilles.
14. A method for isolating cimiracemate A from a Cimicifuga species, comprising the steps of:
a) providing a sufficient quantity of raw material of a Cimicifuga species;
b) mixing the raw material of a Cimicifuga species with water as the solvent at a temperature of 20° C. to 28° C. to obtain a water extract comprising cimiracemate A; and
c) isolating cimiracemate A from the water extract to yield a liquid or dried composition comprising cimiracemate A.
15. The method of claim 14 , wherein the raw material of a Cimicifuga species is ground into powder.
16. The method of claim 14 , wherein the Cimicifuga species is selected from the group consisting of Cimicifuga racemosa, Cimicifuga foetida , and Cimicifuga heracleifolia.
17. The method of claim 16 , wherein the Cimicifuga species is Cimicifuga racemosa.
18. The method of claim 14 , wherein the Cimicifuga species is mixed with water at a ratio of about 1:15 to about 1:20 (w/v).
19. The method of claim 17 , wherein Cimicifuga racemosa is mixed with water at a ratio of about 1:15 (w/v).
20. A method for preparing a composition comprising the isolated cimiracemate A from claim 14 , further comprising formulating the isolated cimiracemate A into a composition selected from the group consisting of tablets, capsules, powders, pastilles, suppositories, oral solutions, suspensions, and injectable compositions.
21. The method of claim 20 , comprising formulating the isolated cimiracemate A into a composition selected from the group consisting of tablets, capsules, powder, and pastilles.