Method for culturing stem cells
In the field of biological technology, a stem cell culture method is provided. The method includes preparing an amniotic epithelial cell feeder layer that is not treated to lose the division ability; and seeding the stem cells onto the amniotic epithelial cell feeder layer, and culturing in a culture medium. The stem cell culture method according to the present invention does not require the treatment of the feeder layer cells to lose the division ability, and is thus simple and safe, thereby effectively solving the problem of contamination caused by animal-derived ingredients in culture of human stem cells at present, greatly reducing the culture cost of the stem cells, and providing a safe, effective, and inexpensive stem cell culture method for the industrialization of the stem cells in the future.
1. A stem cell culture method, comprising:
a) preparing an amniotic epithelial cell feeder layer that is not treated to lose the division ability; and
b) seeding stem cells onto the amniotic epithelial cell feeder layer, and culturing in a culture medium.
2. The stem cell culture method according to claim 1 , wherein step (a) of claim 1 comprises peeling off the amnion from the placenta obtained after parturition through cesarean section, isolating amniotic epithelial cells, seeding the amniotic epithelial cells into a cell culture container, and culturing in a cell incubator at 37° C. with 5% CO 2 .
3. The stem cell culture method according to claim 1 , wherein the amniotic epithelial cells are mammalian amniotic epithelial cells.
4. The stem cell culture method according to claim 3 , wherein the amniotic epithelial cells are human amniotic epithelial cells.
5. The stem cell culture method according to claim 1 , wherein the amniotic epithelial cells are amniotic epithelial cells of passages P 0 -P 3 .
6. The stem cell culture method according to claim 1 , wherein the amniotic epithelial cells are amniotic epithelial cells of passage P 0 .
7. The stem cell culture method according to claim 1 , wherein the stem cells are embryonic stem cells, embryonic germ cells, induced pluripotent stem (iPS) cells, or somatic stem cells.
8. The stem cell culture method according to claim 1 , wherein the stem cells are embryonic stem cells or iPS cells.
9. The stem cell culture method according to claim 8 , wherein the embryonic stem cells or iPS cells are human embryonic stem cells or human iPS cells.
10. The stem cell culture method according to claim 1 , wherein the stem cells are embryonic stem cells, embryonic germ cells, or iPS cells, and in the culture medium comprises 0-12 ng/ml of LIF.
11. The stem cell culture method according to claim 1 , wherein the culture medium is a serum-free culture medium or a serum-containing culture medium.
12. The stem cell culture method according to claim 11 , wherein the serum is human whole serum, umbilical cord serum, animal serum, or animal embryonic serum.