Method for preparing an invasive test of an egg and for determining a gender of an embryo in an egg

1. A method for preparing an invasive test on an egg having a shell, the method comprising:

generating a passage through the shell to allantoic fluid of an egg;

sealing the passage by arranging a seal on the shell at an entrance of the passage, wherein the seal comprises a biomarker and an adhesive.

2. The method of claim 1 , wherein the seal comprises a plug comprising plaster.

3. The method of claim 1 , wherein the biomarker comprises a fluorescent biomarker.

4. The method of claim 1 , further comprising the steps of:

allowing a reaction to occur between the seal and the allantoic fluid of the egg; and

assessing an intensity of the reaction, wherein the intensity of the reaction is a measure for the concentration of testosterone, oestrogen, or a combination thereof in the allantoic fluid of the egg.

5. The method of claim 4 , wherein the reaction is in an interior of the egg shell.

6. The method of claim 1 , wherein the egg is a chicken egg.

7. The method of claim 1 , further comprising the step of:

waiting between 13 and 17 days after conception before providing the passage.

8. The method of claim 1 , further comprising of providing the passage using a laser by directing the laser toward a side of the egg and thereby burning the passage in the shell of the egg.

9. The method of claim 4 , wherein assessing the reaction comprises determining a gender of an embryo in the egg.

10. The method of claim 1 , wherein the invasive test is a test for determining a gender of an embryo in the egg.

11. The method of claim 4 , further comprising determining a gender of an embryo in the egg based on the intensity of the reaction.

12. The method of claim 4 , wherein the reaction is between the biomarker and testosterone, oestrogen, or a combination thereof.

13. The method of claim 1 , wherein arranging the seal on the passage comprises arranging the seal in the passage.