Use of metal complexes
A method of immobilizing a target molecule on a substrate, which comprises exposing the target molecule to the substrate in the presence of a metal complex, wherein the target molecule is an unmodified target molecule, and wherein the metal complex is selected to provide a stable binding interaction between the target molecule and the substrate.
1. A synthetic substrate having a surface for detection of an analyte in a sample, said surface including:
a target molecule for binding to an analyte in a sample, thereby enabling detection of said analyte in said sample, wherein said target molecule has not been modified to facilitate binding with a metal ion;
a metal ion having co-ordination sites bound to said target molecule and said surface, thereby binding said target molecule with said surface;
a co-ordination ligand in the form of an additive ligand or an anion, said co-ordination ligand selected for enhancing or weakening the binding affinity of said metal ion for said surface or said target molecule,
wherein said metal is chromium and said additive ligand is an amine containing molecule.
2. The substrate of claim 1 wherein said additive ligand is ethylenediamine, tetramethylethylenediamine, iminodiacetic acid, nitrilotriacetic acid, 1,10-phenanthroline or 8-hydroxyquinoline.
3. The substrate of claim 1 wherein said chromium has an oxidation state of III.
4. The substrate of claim 1 wherein said substrate takes the form of a bead, membrane, multi-well plate, slide, or capillary column.
5. The substrate of claim 1 wherein said substrate is a carboxylic acid functionalised, amide functionalised, amine functionalised or ester functionalised substrate.
6. The substrate of claim 1 wherein said target molecule is a protein.
7. The substrate of claim 6 wherein said protein is an antibody.