Compositions and methods comprising C16:1n7-palmitoleate
One embodiment described herein is related to methods and compositions, such as nutraceutical formulations and dietary supplements, comprising C16:1n7-palmitoleate or derivatives thereof. The methods and compositions comprising C16:1n7-palmitoleate, or derivatives thereof, safely and effectively prevent or mitigate manifestations of cardiovascular disease, including coronary artery disease and the accumulation of cholesterol or lipid deposits in the blood vessels of a subject.
1. A dietary supplement comprising a C16:1n7-palmitoleate derivative and a palmitate derivative; wherein the supplement
i) has a ratio of the C16:1n7-palmitoleate derivative to the palmitate derivative of 20:1 to 100:1; and
(ii) comprises at least 20 wt % of the C16:1n7-palmitoleate derivative; and
each palmitoleate and palmitate derivative is independently a free acid, pharmaceutically acceptable salt, (C 1 -C 4 )alkyl ester, monoglyceride, diglyceride, triglyceride, or a combination of any two or more thereof.
2. The dietary supplement of claim 1 , comprising at least 35 wt % of the C16:1n7-palmitoleate derivative.
3. The dietary supplement of claim 1 , comprising at least 50 wt % of the C16:1n7-palmitoleate derivative.
4. The dietary supplement of claim 1 , comprising at least 90 wt % of the C16:1n7-palmitoleate derivative.
5. The dietary supplement of claim 1 , wherein the ratio of the C16:1n7-palmitoleate derivative to the palmitate derivative is 20:1 to 50:1.
6. The dietary supplement of claim 1 , wherein the ratio of the C16:1n7-palmitoleate derivative to the palmitate derivative is 50:1 to 100:1.
7. The dietary supplement of claim 1 , wherein all of the derivatives are (C 1 -C 4 )alkyl esters.
8. The dietary supplement of claim 1 , wherein all of the derivatives are ethyl esters.
9. The dietary supplement of claim 1 , wherein all of the derivatives are methyl esters.
10. The dietary supplement of claim 1 , wherein all of the derivatives are free acids or pharmaceutically acceptable salts thereof.
11. The dietary supplement of claim 1 , wherein all of the derivatives are monoglycerides, diglycerides, triglycerides or a combination of any two or more thereof.
12. The dietary supplement of claim 1 , wherein the C16:1n7-palmitoleate derivative is a cis-C16:1n7-palmitoleate derivative.
13. The dietary supplement of claim 1 , wherein the supplement further comprises an oleate derivative, wherein the supplement has a ratio of the C16:1n7-palmitoleate derivative to the oleate derivative of 6:1 to 100:1, and each oleate derivative is independently a free acid, pharmaceutically acceptable salt, (C 1 -C 4 )alkyl ester, monoglyceride, diglyceride, triglyceride or a combination of any two or more thereof.
14. The dietary supplement of claim 13 , wherein the ratio of the C16:1n7-palmitoleate derivative to the oleate derivative is 10:1 to 20:1.
15. The dietary supplement of claim 13 , wherein the ratio of the C16:1n7-palmitoleate derivative to the oleate derivative is 20:1 to 50:1.
16. The dietary supplement of claim 13 , wherein the ratio of the C16:1n7-palmitoleate derivative to the oleate derivative is 50:1 to 100:1.
17. The dietary supplement of claim 1 , wherein the C16:1n7-palmitoleate derivative comprises a C16:1n7-palmitoleate moiety that is obtained from fish.
18. The dietary supplement of claim 17 , wherein the fish are anchovies, menhaden, pollock, herring, cod, salmon, smelt, tuna, mackerel, krill or a combination of any two or more thereof.
19. The dietary supplement of claim 17 , wherein the fish comprise anchovies.
20. The dietary supplement of claim 17 , wherein the fish comprise menhaden.