Herpes simplex viruses and methods of viral replication
A herpes simplex virus is disclosed in which the herpes simplex virus genome comprises a nucleic acid sequence encoding an ING4 polypeptide.
1. A method of replicating herpes simplex virus (HSV) in vitro, the method comprising replicating HSV in vitro in cells having ING4 polypeptide in excess of the amount normally present from endogenous expression of said cells wherein the ING4 polypeptide has at least 95% sequence identity to SEQ ID NO: 3.
2. The method of claim 1 , wherein the cell(s) express ING4 polypeptide from a vector transfected into the cell(s).
3. The method of claim 1 , wherein the cell(s) express ING4 polypeptide from a heterologous construct comprising a nucleic acid sequence encoding an ING4 polypeptide.
4. The method of claim 1 , wherein the cell(s) express ING4 polypeptide from a heterologous construct comprising a nucleic acid sequence encoding an ING4 polypeptide operably linked to a regulatory nucleotide sequence.
5. The method of claim 3 , wherein expression of said nucleic acid sequence encoding ING4 polypeptide is constitutive.
6. The method of claim 1 , wherein the cell(s) overexpress endogenous ING4 polypeptide.
7. The method of claim 1 , wherein the HSV is selected from the group consisting of an HSV-1, HSV-1 strain 17, HSV1716, HSV1716 ING4, HSV-1 strain F, and HSV-1 strain F mutant G207.
8. A method for the in vitro replication of herpes simplex virus (HSV), the method comprising infecting a cell or cells with HSV, culturing the cell(s) in vitro, and harvesting viral progeny from the cell(s), wherein the cells have ING4 polypeptide in excess of the amount normally present from endogenous expression of said cells, and wherein the ING4 polypeptide has at least 95% sequence identity to SEQ ID NO:3.
9. The method of claim 8 , wherein the cell(s) express ING4 polypeptide from a vector transfected into the cell(s).
10. The method of claim 8 , wherein the cell(s) express ING4 polypeptide from a heterologous construct comprising a nucleic acid sequence encoding an ING4 polypeptide.
11. The method of claim 8 , wherein the cell(s) express ING4 polypeptide from a heterologous construct comprising a nucleic acid sequence encoding an ING4 polypeptide operably linked to a regulatory nucleotide sequence.
12. The method of claim 10 , wherein expression of said nucleic acid sequence encoding ING4 polypeptide is constitutive.
13. The method of claim 8 , wherein the cell(s) overexpress endogenous ING4 polypeptide.
14. The method of claim 8 , wherein the HSV is selected from the group consisting of an HSV-1, HSV-1 strain 17, HSV1716, HSV1716ING4, HSV-1 strain F, and HSV-1 strain F mutant G207.
15. A cell, in vitro, infected with a herpes simplex virus, which cell has ING4 polypeptide in excess of the amount normally present from endogenous expression of said cells, wherein the ING4 polypeptide has at least 95% sequence identity to SEQ ID NO:3.
16. A cell according to claim 15 , wherein the cell expresses ING4 polypeptide from a heterologous construct comprising a nucleic acid sequence encoding an ING4 polypeptide.
17. A cell according to claim 15 , wherein the cell overexpresses endogenous ING4 polypeptide.