Plant regulatory elements and uses thereof
The present invention provides novel DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.
1. A DNA molecule comprising a DNA sequence selected from the group consisting of:
a) a sequence comprising SEQ ID NO: 8; and
b) a fragment of SEQ ID NO: 8 comprising SEQ ID NO: 10, 12, 14 or 16, wherein the fragment has gene-regulatory activity;
wherein said sequence is operably linked to a heterologous transcribable polynucleotide molecule.
2. The DNA molecule of claim 1 , wherein the heterologous transcribable polynucleotide molecule comprises a gene of agronomic interest.
3. The DNA molecule of claim 2 , wherein the gene of agronomic interest confers herbicide tolerance in plants.
4. The DNA molecule of claim 2 , wherein the gene of agronomic interest confers pest resistance in plants.
5. A transgenic plant cell comprising a heterologous DNA molecule comprising a sequence selected from the group consisting of:
a) a sequence comprising SEQ ID NO: 8; and
b) a fragment of SEQ ID NO: 8 comprising SEQ ID NO: 10, 12, 14, or 16, wherein the fragment has gene-regulatory activity;
wherein said sequence is operably linked to a heterologous transcribable polynucleotide molecule.
6. The transgenic plant cell of claim 5 , wherein said transgenic plant cell is a monocotyledonous plant cell.
7. The transgenic plant cell of claim 5 , wherein said transgenic plant cell is a dicotyledonous plant cell.
8. A transgenic plant, or part thereof, comprising the DNA molecule of claim 1 .
9. A progeny plant of the transgenic plant of claim 8 , or a part thereof, wherein the progeny plant or part thereof comprises said DNA molecule.
10. A transgenic seed, wherein the seed comprises the DNA molecule of claim 1 .
11. A method of producing a commodity product comprising obtaining a transgenic plant or part thereof according to claim 8 and producing the commodity product therefrom.
12. The method of claim 11 , wherein the commodity product is protein concentrate, protein isolate, grain, starch, seeds, meal, flour, biomass, or seed oil.
13. A commodity product comprising the DNA molecule of claim 1 .
14. The commodity product of claim 13 , wherein the commodity product is protein concentrate, protein isolate, grain, starch, seeds, meal, flour, biomass, or seed oil.
15. A method of expressing a transcribable polynucleotide molecule comprising obtaining a transgenic plant according to claim 8 and cultivating said plant, wherein the transcribable polynucleotide is expressed.