Compositions for solubilizing cells and/or tissue
Solubilizing compositions are provided that include a 3-(alkyl dimethyl ammonia) propane sulfonate zwitterionic surfactant and a polyethylene glycol alkyl ether nonionic surfactant, provided that the zwitterionic surfactant and the nonionic surfactant are not simultaneously and respectively 3-(decyl dimethyl ammonia) propane sulfonate and polyoxyethylene (4) lauryl ether. Also provided are methods for solubilizing cells and/or tissue of a subject in vivo and methods for recovering proteins from skin cells of a subject in vivo using the solubilizing compositions.
1. A method for solubilizing skin cells and/or skin tissue of a subject in vivo, the method comprising:
contacting the skin cells and/or skin tissue of the subject in vivo with a buffered composition effective to solubilize the skin cells and/or skin tissue of the subject in vivo, the buffered composition comprising:
a 3-(alkyl dimethyl ammonia) propane sulfonate zwitterionic surfactant; and
a polyethylene glycol alkyl ether nonionic surfactant,
the buffered composition being characterized by a pH of between about 7 and about 9,
provided that the zwitterionic surfactant and the nonionic surfactant are not simultaneously and respectively 3-(decyl dimethyl ammonia) propane sulfonate and polyoxyethylene (4) lauryl ether.
2. The method of claim 1 , further comprising subjecting the skin cells and/or skin tissue to energy.
3. The method of claim 1 , further comprising collecting from the solubilized skin cells and/or skin tissue one or more of: a small organic molecule, a protein, DNA, RNA, and a lipid.
4. The method of claim 1 , further comprising collecting at least one of cytosolic proteins, nuclear proteins, and surface proteins from the solubilized skin cells and/or skin tissue.
5. The method of claim 1 , wherein the buffered composition further comprises a protease inhibitor.
6. The method of claim 1 , wherein the skin cells comprise at least one of endothelial cells and keratinocyte cells.
7. The method of claim 1 , the buffered composition comprising one or more of: phosphate-buffered saline, Tris-buffered saline, Tris-hydrochloride, and ethylene diamine tetraacetic acid.
8. The method of claim 1 , wherein the composition further comprises a buffer solution, the pH of the composition in the buffer solution being about 8.8.
9. The method of claim 1 , the buffered composition characterized by a total (w/v) concentration of the zwitterionic and nonionic surfactants of one or more of: between about 0.01% and about 10%; between about 0.01% and about 5%; between about 0.1% and about 2%; between about 0.1% and about 0.5%; and about 1%.
10. The method of claim 1 , the buffered composition characterized by a concentration ratio of the zwitterionic surfactant to the nonionic surfactant of between about 2:3 and about 3:2.
11. A method for recovering proteins from skin cells of a subject in vivo, the method comprising:
contacting the skin cells of the subject in vivo with a buffered composition effective to provide solubilized proteins from the skin cells of the subject in vivo, the buffered composition comprising:
a 3-(alkyl dimethyl ammonia) propane sulfonate zwitterionic surfactant; and
a polyethylene glycol alkyl ether nonionic surfactant, the buffered composition being characterized by a pH of between about 7 and about 9, provided that the zwitterionic surfactant and the nonionic surfactant are not simultaneously and respectively 3-(decyl dimethyl ammonia) propane sulfonate and polyoxyethylene (4) lauryl ether; and
subjecting the solubilized proteins to a reverse phase protein array.
12. The method of claim 11 , wherein the buffered composition further comprises an alkyl sulfate surfactant or a salt thereof.
13. The method of claim 11 , wherein the solubilized proteins comprise signaling proteins.
14. The method of claim 11 , further comprising performing unsupervised hierarchical clustering to identify the solubilized proteins.
15. The method of claim 11 , the buffered composition characterized by a total (w/v) concentration of the zwitterionic and nonionic surfactants of one or more of: between about 0.01% and about 10%; between about 0.01% and about 5%; between about 0.1% and about 2%; between about 0.1% and about 0.5%; and about 1%.
16. The method of claim 11 , the buffered composition characterized by a concentration ratio of the zwitterionic surfactant to the nonionic surfactant of between about 2:3 and about 3:2.