Acid fungal proteases
The present invention is directed to novel acid proteases and more specifically to NSP24 family proteases and NSP25 family proteases including biologically active fragments thereof and to nucleic acid molecules encoding said proteases. Also provided are vectors and host cells including nucleic acid sequences coding for the proteases, methods for producing the proteases, enzyme compositions and methods employing said proteases.
1. An isolated polynucleotide encoding an NSP25 family protease having at least 97% sequence identity to the polypeptide sequence of SEQ ID NO:9.
2. The isolated polynucleotide of claim 1 having the nucleotide sequence of SEQ ID NO:4.
3. A vector comprising the polynucleotide of claim 1 .
4. An isolated host cell transformed with the polynucleotide of claim 1 .
5. The host cell of claim 4 , wherein the host cell is a filamentous fungal cell.
6. The host cell of claim 5 , wherein the filamentous fungal cell is an Aspergillus spp., a Fusarium spp., or a Trichoderma spp.
7. The host cell of claim 6 , wherein the Aspergillus is A. niger, A. oryzae, A. nidulans , or A. awamori.
8. The host cell of claim 6 , wherein the Trichoderma is T. reesei.
9. The host cell of claim 6 , wherein the Trichoderma host cell comprises deletions of at least two endoducanase-encoding genes and at least two cellobiohydrolase-encoding genes.
10. A method for producing a protease comprising
a) introducing into an isolated host cell a polynucleotide comprising a promoter operably linked to a nucleic acid encoding a NSP25 family protease having at least 97% sequence identity to the polypeptide sequence of SEQ ID NO:9,
b) culturing the host cell under suitable culture conditions for the expression and production of the NSP25 family protease, and
c) producing said NSP25 family protease.