IP Library › Granted Patent US 10,227,569
Granted Patent B2
US 10,227,569 · App. 13/444,338 · Granted Mar 12, 2019

Respiratory syncytial virus expression vectors

Inventors: Martin L. Moore (Decatur, GA); Anne Hotard (Atlanta, GA)
Assignees: EMORY UNIVERSITY; CHILDREN'S HEALTHCARE OF ATLANTA, INC.
C12N7/00C12N15/86C12N2760/18543C12N2760/18552C12N2800/204C12N2800/50
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Quick Facts
Patent No.
US 10,227,569
App. No.
13/444,338
Granted
Mar 12, 2019
Kind
B2
Abstract

In certain embodiments, the disclosure relates to vectors containing bacterial nucleic acid sequences and a paramyxovirus gene. Typically, the expression vector comprises a bacterial artificial chromosome (BAC), and a nucleic acid sequence comprising a respiratory syncytial virus (RSV) gene in operable combination with a regulatory element and optionally a reporter gene.

Claims (16)

1. A vector comprising

a) a bacterial artificial chromosome comprising oriS, repE, parA, and parB genes of Factor F, and

b) a nucleic acid sequence comprising a respiratory syncytial virus (RSV) antigenome

wherein the vector comprises restriction sites BstBI, SacI, ClaI, and MluI in that order.

2. The vector of claim 1 , wherein the nucleic acid sequence comprising the RSV sequence is a strain that is optionally mutated.

3. The vector of claim 1 , wherein the bacterial artificial chromosome comprises a selectable marker.

4. An isolated bacteria comprising the vector of claim 1 .

5. An isolated cell comprising the vector of claim 1 .

6. The isolated cell of claim 5 , further comprising a vector selected from the group consisting of a vector encoding an N protein of RSV, a vector encoding a P protein of RSV, a vector encoding an L protein of RSV, and a vector encoding an M2-1 protein of RSV.

7. The isolated cell of claim 6 , wherein the vector comprises a promoter and the isolated cell encodes a polypeptide that activates transcription downstream of the promoter.

8. The isolated cell of claim 7 , wherein the promoter is T7.

9. The isolated cell of claim 7 , wherein the polypeptide that activates transcription downstream of the promoter is T7 RNA polymerase.

10. A vector comprising

a) a bacterial artificial chromosome comprising oriS, repE, parA, and parB genes of Factor F, and

b) a nucleic acid sequence comprising a respiratory syncytial virus (RSV) antigenome in operable combination with a reporter gene

wherein the vector comprises restriction sites BstBI, SacI, ClaI, and MluI in that order.

Assignments (1)
CONFIRMATORY LICENSE Recorded Jun 26, 2013
From: EMORY UNIVERSITY
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 030686/0839 →
Continuity (2)
Provisional Application 61474365 · Apr 12, 2011
Related Publication 20120264217A1 · Oct 18, 2012