BGL6 beta-glucosidase and nucleic acids encoding the same
The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl6, and the corresponding BGL6 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL6, recombinant BGL6 proteins and methods for producing the same.
1. A substantially purified β-glucosidase 6 (BGL6) polypeptide with the enzymatic activity of a β-glucosidase, comprising a sequence selected from the group consisting of:
(a) an amino acid sequence having at least 95% sequence identity to the amino acid sequence presented as SEQ ID NO:2;
(b) the amino acid sequence presented as SEQ ID NO:2; and
(c) a substantially purified enzymatically active fragment of the amino acid sequence presented as SEQ ID NO:2.
2. A detergent composition, said composition comprising a polypeptide selected from the group consisting of:
(a) an amino acid sequence having at least 95% sequence identity to the amino acid sequence presented as SEQ ID NO:2;
(b) an amino acid sequence presented as SEQ ID NO:2;
(c) a substantially purified enzymatically active fragment of the amino acid sequence presented as SEQ ID NO:2.
3. The BGL6 polypeptide of claim 1 , wherein said polypeptide is expressed in a host cell.
4. The BGL6 polypeptide of claim 3 wherein said host cell is a filamentous fungus or a yeast cell.
5. The BGL6 polypeptide of claim 4 , wherein said filamentous fungus cell is a cell of the group selected from Trichoderma sp.; Penicillium sp., Humicola sp., including Humicola insolens; Aspergillus sp., Chrysosporium sp., Fusarium sp., Hypocrea sp., and Emericella sp.
6. The BGL6 polypeptide of claim 3 , wherein said host cell is genetically modified.
7. The BGL6 polypeptide of claim 6 , wherein said modified host cell is modified to achieve an increase in BGL6 expression, a decrease or elimination in BGL6 expression, or expression of an altered form of BGL6.
8. The BGL6 polypeptide of claim 3 , wherein said polypeptide is native or heterologous to the host cell.
9. A BGL6 polypeptide produced by fermentation of the modified host cell of claim 6 .
10. A cellulase composition comprising a polypeptide of claim 1 .
11. The cellulase composition of claim 10 , wherein said polypeptide is present at about 1 to about 25 weight percent relative to any cellobiohydrolase or endoglucanase type components present in the cellulase composition.
12. The cellulase composition of claim 10 , wherein said composition is a fermentation culture.