Patent
Disclosed herein are microbiocidal compositions that include spore-forming strains of Bacillus . The compositions may be utilized to treat or prevent disease in aquatic animals such as farmed fish or crustaceans.
1. A pelleted feed composition for aquatic animals, wherein the feed is provided with a coating and the feed composition comprises a spore-forming strain of Bacillus having a 16S rDNA sequence comprising SEQ ID NO: 1, SEQ ID NO: 2, or SEQ ID NO: 3, or comprising a 16S rDNA sequence having at least 99% sequence identity to SEQ ID NO: 1, SEQ ID NO: 2, or SEQ ID NO: 3, wherein the spore-forming strain of the Bacillus is present at a concentration of at least 10 4 CFU/g of the feed composition.
2. The feed composition of claim 1 , wherein the spore-forming strain of the Bacillus is a Bacillus species having a 16S rDNA comprising SEQ ID NO: 1, SEQ ID NO: 2, or SEQ ID NO: 3.
3. The feed composition of claim 1 , wherein the spore-forming strain of the Bacillus is susceptible to one or more antibiotics selected from the group consisting of carbenicillin, ampicillin, spectinomycin, oxacillin, vancomycin, cephalothin, novobiocin, sulfadiazine, amikacin, erythromycin, neomycin, penicillin, chloramphenicol, sulfamethoxazole, norfloxacin, gentamicin and ciprofloxacin.
4. The feed composition of claim 1 , wherein the feed composition is a feed composition for farmed fish.
5. The feed composition of claim 1 , wherein the spore-forming strain of the Bacillus is the single strain of Bacillus present in the feed composition.
6. The feed composition of claim 1 , wherein the composition comprises a mixture of spore-forming strains of Bacillus.
7. The feed composition of claim 1 , wherein the spore-forming strain of the Bacillus is selected from the group consisting of Bacillus strains deposited under the accession numbers NRRL B-50745, NRRL B-50741, NRRL B-50742, NRRL B-50743, and NRRL B-50744.
8. A pelleted feed composition for aquatic animals, wherein the feed is provided with a coating and the feed composition comprises a spore-forming strain of Bacillus , wherein the spore-forming strain of Bacillus is a Bacillus species having a 16S rDNA comprising SEQ ID NO: 1, SEQ ID NO: 2, or SEQ ID NO: 3, and the spore-forming strain of the Bacillus is present at a concentration of at least 10 4 CFU/g of the feed composition.
9. The feed composition of claim 8 , wherein the spore-forming strain of the Bacillus inhibits the growth of one or more bacteria selected from the group consisting of Aeromonas hydrophila, Flavobacterium columnare, Edwardsiella ictaluri, Edwardsiella tarda, Streptococcus iniae , and Yersinia ruckeri.
10. A pelleted feed composition for aquatic animals, wherein the feed is provided with a coating and the feed composition comprises a spore-forming strain of Bacillus selected from the group consisting of Bacillus strains deposited under the accession numbers NRRL B-50745, NRRL B-50741, NRRL B-50742, NRRL B-50743, and NRRL B-50744, and wherein the spore-forming strain of the Bacillus is present at a concentration of at least 10 4 CFU/g of the feed composition.