Zwitterionic polymer bioconjugates and related methods
View Patent ↗Zwitterionic polymer and mixed charge copolymer bioconjugates, methods for making and using the bioconjugates.
1. A zwitterionic polymer bioconjugate comprising one or more zwitterionic polymers covalently coupled to a biomolecule, wherein the zwitterionic polymer comprises a plurality of repeating units, each repeating unit having the formula:
wherein
R 1 is selected from the group consisting of hydrogen, fluorine, trifluoromethyl, C1-C6 alkyl, and C6-C12 aryl groups;
R 2 and R 3 are independently selected from the group consisting of alkyl and aryl, and taken together with the nitrogen to which they are attached form a cationic center;
L 1 is a linker that covalently couples the cationic center [N + (R 2 )(R 3 )] to the polymer backbone [—(CH 2 —CR 1 ) n —];
L 2 is a linker that covalently couples the anionic center [A(=O)O − ] to the cationic center;
A is C;
M + is a counterion associated with the (A=O)O − anionic center;
X − is a counter ion associated with the cationic center;
n is an integer from 1 to about 10,000; and
the biomolecule is a protein, a nucleic acid, a glycoprotein, a proteoglycan, or a lipid.
2. The conjugate of claim 1 , wherein R 1 , R 2 , and R 3 are independently selected from the group consisting of C1-C3 alkyl.
3. The conjugate of claim 1 , wherein L 1 is selected from the group consisting of —C(═O)O—(CH) n′ and —C(═O)NH—(CH 2 ) n —, wherein n′ is an integer from 1 to 20.
4. The conjugate of claim 1 , wherein L 2 is —(CH 12 ) n″ —, where n″ is an integer from 1 to 20.
5. A composition, comprising the conjugate of claim 1 and a pharmaceutically accepted carrier or diluent.