Chemically-defined arrays for screening cell-substrate interactions
Patterned SAM arrays and methods of preparing patterned SAM arrays are disclosed. Advantageously, the methods used to prepare the patterned SAM arrays allow for controlling SAM spot-to-spot conditions such as ligand identity and ligand density, which allows for preparing a wide range of SAM spots in a single array format. Additionally, the patterned SAM arrays of the present disclosure support the culture of a range of cell types. The patterned SAM arrays offer the ability to rapidly screen substrate components for influencing cell attachment, spreading, proliferation, migration, and differentiation.
1. A method for preparing a self-assembled monolayer array comprising:
adhering a polymer stencil to a metal-coated substrate, wherein the polymer stencil comprises at least one well;
forming at least one alkanethiolate self-assembled monolayer spot on the metal-coated substrate, wherein the alkanethiolate self-assembled monolayer spot is formed in the at least one well of the polymer stencil;
removing the polymer stencil from the metal-coated substrate; and
backfilling a region on the metal-coated substrate that surrounds the alkanethiolate self-assembled monolayer spot, wherein the backfilling forms an alkanethiolate self-assembled monolayer surrounding the at least one alkanethiolate self-assembled monolayer spot.
2. The method of claim 1 , further comprising immobilizing a ligand on the alkanethiolate self-assembled monolayer spot.
3. The method of claim 2 , wherein the ligand is immobilized to an alkanethiol prior to forming the at least one alkanethiolate self-assembled monolayer spot.
4. The method of claim 2 , wherein the ligand is immobilized using at least one of carbodiimide chemistry, azide-alkyne cycloaddition chemistry, and maleimide chemistry.
5. The method of claim 2 , wherein the ligand is selected from the group consisting of a protein, a nucleic acid, a polysaccharide, a lipid, and combinations thereof.
6. The method of claim 1 , further comprising immobilizing a ligand to the alkanethiolate self-assembled monolayer surrounding the alkanethiolate self-assembled monolayer spot.
7. The method of claim 1 , wherein the metal comprises gold, titanium, copper, stainless steel, silver, platinum, ruthenium, rhodium, palladium, osmium, iridium and combinations thereof.